HIPK3 Blocking Peptide for STJ501346 is synthetically produced from the 860-910 sequence and is suitable for use in western blot applications.
Applications
Immunodepletion/Immunocompetition
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Formulation
Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice.
Storage Instruction
Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Synthetic peptide taken within amino aci region 860-910 on human Homeodomain-interacting protein kinase 3 protein.
Immunogen Region
860-910
Specificity
This blocking peptide is recommended for use in combination with HIPK3 antibody, STJ501346
Additional Info
Tissue Specificity
Overexpressed in multidrug resistant cells. Highly expressed in heart and skeletal muscle, and at lower levels in placenta, pancreas, brain, spleen, prostate, thymus, testis, small intestine, colon and leukocytes. Not found in liver and lung.
Post Translational Modifications
Autophosphorylated, but autophosphorylation is not required for catalytic activity. May be sumoylated.
Function
Serine/threonine-protein kinase involved in transcription regulation, apoptosis and steroidogenic gene expression. Phosphorylates JUN and RUNX2. Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors. The phosphorylation of NR5A1 activates SF1 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. In osteoblasts, supports transcription activation: phosphorylates RUNX2 that synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE).
Peptide Name
Homeodomain-Interacting Protein Kinase 3Androgen Receptor-Interacting Nuclear Protein KinaseAnpkFas-Interacting Serine/Threonine-Protein KinaseFistHomolog Of Protein Kinase Yak1
Cellular Localisation
CytoplasmNucleus
Alternative Peptide Names
Homeodomain-Interacting Protein Kinase 3 proteinAndrogen Receptor-Interacting Nuclear Protein Kinase proteinAnpk proteinFas-Interacting Serine/Threonine-Protein Kinase proteinFist proteinHomolog Of Protein Kinase Yak1 proteinHIPK3 proteinDYRK6 proteinFIST3 proteinPKY protein
