GPS2 Positive Control for STJ501285 peptide (STJ505644)

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STJ505644-5

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Applications: WB
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: GPS2 Positive Control for STJ501285 is synthetically produced from the sequence and is suitable for use in western blot applications.
Formulation: Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is
Dilution Range: WB: 1:2, 500-1:5, 000
Storage Instruction: Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Gene Symbol: GPS2
Gene ID: 2874
Uniprot ID: GPS2_HUMAN
Specificity: This is positive control is recommended for use in combination with GPS2 antibody STJ501285.
Immunogen: Purfied protein.
Post Translational Modifications Sumoylation regulates its subcellular location. Sumoylation at Lys-45 and Lys-71 regulates the shuttling between the cytoplasm and the nucleus. Sumoylation at Lys-71 is required for interaction with TBL1X. Sumoylated at Lys-45 and Lys-71 in mitochondrion. Desumoylation by SENP1 leads to relocation from the mitochondria to the nucleus. Ubiquitinated at the C-terminus by SIAH2.leading to its degradation by the proteasome. Interaction with TBL1X and methylation at Arg-323 protect GPS2 against ubiquitination and degradation. Methylated at Arg-312 and Arg-323 by PRMT6. Methylation at Arg-323 protects from degradation by the proteasome.
Function Key regulator of inflammation, lipid metabolism and mitochondrion homeostasis that acts by inhibiting the activity of the ubiquitin-conjugating enzyme UBE2N/Ubc13, thereby inhibiting 'Lys-63'-linked ubiquitination. In the nucleus, can both acts as a corepressor and coactivator of transcription, depending on the context. Acts as a transcription coactivator in adipocytes by promoting the recruitment of PPARG to promoters: acts by inhibiting the activity of the ubiquitin-conjugating enzyme UBE2N/Ubc13, leading to stabilization of KDM4A and subsequent histone H3 'Lys-9' (H3K9) demethylation. Promotes cholesterol efflux by acting as a transcription coactivator. Acts as a regulator of B-cell development by inhibiting UBE2N/Ubc13, thereby restricting the activation of Toll-like receptors (TLRs) and B-cell antigen receptors (BCRs) signaling pathways. Acts as a key mediator of mitochondrial stress response: in response to mitochondrial depolarization, relocates from the mitochondria to the nucleus following desumoylation and specifically promotes expression of nuclear-encoded mitochondrial genes. Promotes transcription of nuclear-encoded mitochondrial genes by inhibiting UBE2N/Ubc13. Can also act as a corepressor as part of the N-Cor repressor complex by repressing active PPARG. Plays an anti-inflammatory role in macrophages and is required for insulin sensitivity by acting as a corepressor. Plays an anti-inflammatory role during the hepatic acute phase response by interacting with sumoylated NR1H2 and NR5A2 proteins, thereby preventing N-Cor corepressor complex dissociation. In the cytosol, also plays a non-transcriptional role by regulating insulin signaling and pro-inflammatory pathways. In the cytoplasm, acts as a negative regulator of inflammation by inhibiting the pro-inflammatory TNF-alpha pathway.acts by repressing UBE2N/Ubc13 activity. In the cytoplasm of adipocytes, restricts the activation of insulin signaling via inhibition of UBE2N/Ubc13-mediated ubiquitination of AKT. Able to suppress G-protein- and mitogen-activated protein kinase-mediated signal transduction. Acts as a tumor-suppressor in liposarcoma. (Microbial infection) Required for efficient replication of hepatitis C virus (HCV) by promoting the interaction between VAPA and HCV virus protein NS5A.
Peptide Name G Protein Pathway Suppressor 2
Gps-2
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-3214815
Reactome: R-HSA-9022537
Reactome: R-HSA-9022692
Reactome: R-HSA-9029569
Reactome: R-HSA-9609690
Cellular Localisation Nucleus
Mitochondrion
Cytoplasm
Cytosol
Sumoylation Regulates The Subcellular Location
Relocates From The Mitochondria To The Nucleus Following Desumoylation
Leading To Mediate Mitochondrial Stress Response
Alternative Peptide Names G Protein Pathway Suppressor 2 protein
Gps-2 protein
GPS2 protein

Information sourced from Uniprot.org

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