| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Gastrin Positive Control is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: |
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Dilution Range: |
WB: 1:500 |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Post Translational Modifications | Two different processing pathways probably exist in antral G-cells. In the dominant pathway progastrin is cleaved at three sites resulting in two major bioactive gastrins, gastrin-34 and gastrin-17. In the putative alternative pathway, progastrin may be processed only at the most C-terminal dibasic site resulting in the synthesis of gastrin-71. Sulfation enhances proteolytic processing, and blocks peptide degradation. Levels of sulfation differ between proteolytically-cleaved gastrins. Thus, gastrin-6 is almost 73% sulfated, whereas the larger gastrins are less than 50% sulfated. Sulfation levels are also tissue-specific. |
| Function | Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine. |
| Peptide Name | Gastrin Cleaved Into - Gastrin-71Gastrin Component I - Gastrin-52G52 - Big GastrinGastrin Component IiGastrin-34G34 - GastrinGastrin Component IiiGastrin-17G17 - Gastrin-14G14 - Gastrin-6G6 |
| Database Links | Reactome: R-HSA-416476Reactome: R-HSA-881907 |
| Cellular Localisation | Secreted |
| Alternative Peptide Names | Gastrin Cleaved Into - Gastrin-71 proteinGastrin Component I - Gastrin-52 proteinG52 - Big Gastrin proteinGastrin Component Ii proteinGastrin-34 proteinG34 - Gastrin proteinGastrin Component Iii proteinGastrin-17 proteinG17 - Gastrin-14 proteinG14 - Gastrin-6 proteinG6 proteinGAST proteinGAS protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
