feline IL-2 (C146S) protein (Recombinant) (His-Tag) (STJP019336)

SPECIFICATIONS
HostE.coli
ImmunogenFeline
STJP019336
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General Information

Short DescriptionRecombinant-feline IL-2 (C146S)-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications.
ApplicationsSDS-PAGE/Bioactivity
HostE.coli
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

Concentration0.25 mg/mL
FormulationLiquid in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% Glycerol
Storage InstructionFor short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles.
Endotoxin< 1 EU per 1ug of protein (determined by LAL method)
ImmunoreactivityMeasured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The ED50 range ≤0.3ng/ml.

Target Information

Accession NumberNP_001036802
ImmunogenFeline
Immunogen Region21-154aa
Immunogen SequenceAPASSST KETQQQLEQL LLDLRLLLNG VNNPENPKLS RMLTFKFYVP KKATELTHLQ CLVEELKPLE EVLYLAQSKN FHLNHIKELM SNINVTVLKL KGSETRFTCN YDDETATIVE FLNKWITFCQ SIFSTLT

Additional Info

Background IL2, also known as interleukin2, is an immunoregulatory lymphokine produced by T-cell in response to antigenic or mitogenic stimulation IL2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated kill cells, monocytes, macrophages and oligodendrocytes. Recombinant feline IL2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Information sourced from Uniprot.org

Citations

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