| Applications: | WB |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | E2F4 Positive Control for STJ500826 is synthetically produced from the 360-410 sequence and is suitable for use in western blot applications. |
| Formulation: | Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Dilution Range: | WB: 1:500 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | E2f4 |
| Gene ID: | 104394 |
| Uniprot ID: | E2F4_MOUSE |
| Immunogen Region: | 360-410 |
| Specificity: | This is positive control is recommended for use in combination with E2F4 antibody STJ500826. |
| Immunogen: | Synthetic peptide taken within amino acid region 360-410 on mouse E2F4 protein. |
| Post Translational Modifications | Differentially phosphorylated in vivo. |
| Function | Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTCCGCGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis. |
| Peptide Name | Transcription Factor E2f4E2f-4 |
| Database Links | Reactome: R-MMU-1538133Reactome: -MMU-2173796Reactome: -MMU-69231 |
| Cellular Localisation | Nucleus |
| Alternative Peptide Names | Transcription Factor E2f4 proteinE2f-4 proteinE2f4 protein |
Information sourced from Uniprot.org