E.coli ansA protein (Recombinant) (His-Tag) (STJP017212)

SPECIFICATIONS
HostE.coli
ImmunogenE.coli
STJP017212
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General Information

Short DescriptionRecombinant-E.coli ansA-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications.
ApplicationsSDS-PAGE
HostE.coli
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

Concentration1 mg/mL
FormulationLiquid in 20mM Tris-HCl buffer (pH 8.0) containing 10% Glycerol, 2mM DTT
Storage InstructionFor short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles.

Target Information

Accession NumberNP_416281
ImmunogenE.coli
Immunogen Region1-338aa
Immunogen Sequence MQKKSIYVAY TGGTIGMQRS EQGYIPVSGH LQRQLALMPE FHRPEMPDFT IHEYTPLMDS SDMTPEDWQH IAEDIKAHYD DYDGFVILHG TDTMAYTASA LSFMLENLGK PVIVTGSQIP LAELRSDGQI NLLNALYVAA NYPINEVTLF FNNRLYRGNR TTKAHADGFD AFASPNLPPL LEAGIHIRRL NTPPAPHGEG ELIVHP

Additional Info

Background AnsA is the cytoplasmic asparaginase from Escherichia coli involved in intracellular asparagine utilization. Escherichia coli possesse two l-asparaginases: the cytoplasmic type I form (ansA) and the periplasmic type II form (ansB). AnsA (Type I asparaginase) is constitutively expressed and required for the growth of the bacteria on asparagine as the sole nitrogen source. Recombinant E. coli ansA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Information sourced from Uniprot.org

Citations

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