| Applications: | WB |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Phospho-Cofilin Positive Control is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | CFL1 |
| Gene ID: | 1072 |
| Uniprot ID: | COF1_HUMAN |
| Tissue Specificity | Widely distributed in various tissues. |
| Post Translational Modifications | Inactivated by phosphorylation on Ser-3. Phosphorylated on Ser-3 in resting cells. Dephosphorylated by PDXP/chronophin.this restores its activity in promoting actin filament depolymerization. The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal. Phosphorylated via a ARRB1-RAC1-LIMK1-PAK1 cascade upon active ligand stimulation of atypical chemokine receptor ACKR2. |
| Function | Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics. Required for the centralization of the mitotic spindle and symmetric division of zygotes. Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. Required for neural tube morphogenesis and neural crest cell migration. |
| Peptide Name | Cofilin-1 18 Kda Phosphoprotein P18 Cofilin - Non-Muscle Isoform |
| Database Links | Reactome: R-HSA-114608 Reactome: R-HSA-2029482 Reactome: R-HSA-3928662 Reactome: R-HSA-399954 Reactome: R-HSA-5627117 Reactome: R-HSA-8950505 |
| Cellular Localisation | Nucleus Matrix Cytoplasm Cytoskeleton Cell Projection Ruffle Membrane Peripheral Membrane Protein Cytoplasmic Side Lamellipodium Membrane Lamellipodium Growth Cone Axon Colocalizes With The Actin Cytoskeleton In Membrane Ruffles And Lamellipodia Detected At The Cleavage Furrow And Contractile Ring During Cytokinesis Almost Completely In Nucleus In Cells Exposed To Heat Shock Or 10% Dimethyl Sulfoxide |
| Alternative Peptide Names | Cofilin-1 protein 18 Kda Phosphoprotein P18 protein Cofilin - Non-Muscle Isoform protein CFL1 protein CFL protein |
Information sourced from Uniprot.org