| Short Description : | Recombinant-canine IL-1 beta/IL1B-No-Tag protein was developed from e.coli and has a target region of No-Tag. For use in research applications. |
| Applications: | SDS-PAGE/Bioactivity |
| Host: | E.coli |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Concentration: | 1 mg/mL |
| Formulation: | Liquid in phosphate-Buffered Saline (pH 7.4) |
| Storage Instruction: | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Endotoxin: | < 1 EU per 1ug of protein (determined by LAL method) |
| Immunoreactivity: | Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 range ≤ 5pg/ml. |
| Accession Number: | NP_001033060.1 |
| Immunogen: | Canine |
| Immunogen Region: | 114-265aa |
| Immunogen Sequence: | MAAMQSVDCK LQDISHKYLV LSNSYELRAL HLNGENVNKQ VVFHMSFVHG DESNNKIPVV LGIKQKNLYL SCVMKDGKPT LQLEKVDPKV YPKRKMEKRF VFNKIEIKNT VEFESSQYPN WYISTSQVEG MPVFLGNTRG GQDITDFTME FSS |
| Background | Interleukin-1 (IL-1) has been considered a potentially important inflammatory mediator. It is composed of two distinct proteins, called as IL-1alpha and IL-1beta. IL-1beta is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta bind to the same receptor and they are structurally related polypeptides that share approximately 20% amino acid. Recombinant canine IL-1beta was expressed in E. coli and purified by using conventional chromatography techniques. |
Information sourced from Uniprot.org
