Trial size CASP4 antibodies available in the Antibody Validation Project

Posted by M.Popov on 12th Nov 2020

Trial size CASP4 antibodies available in the Antibody Validation Project

Trial size CASP4 antibodies available in the Antibody Validation Project

Introduction:

CASP4 is a gene encoding the Caspase 4 protein, which is responsible for the cell execution phase of apoptosis and apart from that it has a crucial role in restricting Salmonella typhimurium replication in epithelial cells. Apart from all the important and beneficial roles this gene and its encoded protein have, they are also responsible for several diseases if they are mutated or overexpressed including cowpox, cystinosis and cancer, including neuroblastoma. We provide a number of trial sized antibodies, which are suitable for CASP4 research purposes, and to help you make the right decision and find the best suited antibody for you, please find the answers to some of the key topics regarding CASP4 that we have provided in the links below.

What is CASP4?

This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. The produced protein is an essential effector of NLRP3 inflammasome-dependent CASP1 activation and IL1B and IL18 secretion in response to non-canonical activators, such as UVB radiation, cholera enterotoxin subunit B and cytosolic LPS and also plays a crucial role in the restriction of Salmonella typhimurium replication in colonic epithelial cells during infection. In later stages of the infection, LPS from cytosolic Salmonella triggers CASP4 activation, which ultimately results in pyroptosis of infected cells and their extrusion into the gut lumen, as well as in IL18 secretion. It is also involved in cell death induced by endoplasmic reticulum stress and by treatment with cytotoxic APP peptides found Alzheimer's patient brains.

Where is CASP4 found?

Inside the cell, CASP4 can be found in the cytosol and the plasma membrane, but on tissue level most tissues show cytoplasmic expression apart from gastro and lymphatic organs which show high membranous expression.

What role does CASP4 play in disease?

When overexpressed, the CASP4 gene induces cell apoptosis and neuroblastoma, which is a type of cancer formed in immature nerve tissue called neuroblasts. Mutations of CASP4 can lead to cardiomyopathy and combined with overexpression it is responsible for cystinosis and a possible role of the protein in the apoptotic loss of proximal tubular cells.

CASP4 antibodies in the Antibody Validation project

St John's Laboratory offers a variety of trial size primary antibodies which target CASP4, which are available to try through the Antibody Validation Project. Below we highlight those which have already been reviewed, as well as others which are available to try.

Customer reviewed CASP4 antibodies

ModelNameHostClonalityRatingApplication Tested
STJ92022Anti-Caspase-4 antibodyRabbitPolyclonal5WB

Other CASP4 antibodies available in the Antibody Validation Project

ModelNameHostClonalityRecommended Applications
STJ150276Mouse CASP4 ELISA Kit  ELISA
STJ90027Anti-Cleaved-Caspase-4 p20 (Q81) antibodyRabbitPolyclonalWB,IHC,ELISA
STJ90028Anti-Cleaved-Caspase-4/5 p20 (D270/D311) antibodyRabbitPolyclonalWB,ELISA
ModelNameHostClonalityRecommended Applications
STJ70593Anti-CASP4 AntibodyGoatPolyclonalELISA,WB

How does the Antibody Validation Project work?

In order to help our customers with their choice of antibody with low risk, we offer one of the largest ranges of primary antibodies with trial size options available.

The Antibody Validation Project works like this.
1. Order your trial size antibody online.
2. Explore its suitability.
3. Return your results by posting them on the product page.
4. Receive a sample refund.

Find out more here.