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Insights, protocols and research news from the St John's Laboratory team. Filter by topic below to find articles relevant to your work.

Top 5 tips: Western Blots

Top 5 tips: Western Blots

21st May 2026

Top 5 Tips for Western Blotting Western blotting is one of the most routine techniques in molecular biology. Most labs run blots weekly. Some run them daily. And yet inconsistent results, unexpected
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Choosing BSA vs Non-Fat Milk

Choosing BSA vs Non-Fat Milk

Posted by S.Davis on 12th Nov 2020

Which is best? BSA vs Non-Fat Milk So, you are about to carry out a western blot. As a researcher you may be asking yourself one of the most common, and appropriate questions there is in regards to s
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Using Rf value to calculate molecular weight

Using Rf value to calculate molecular weight

Posted by J.Money on 12th Nov 2020

Using Rf value to calculate molecular weight In a previous post, we looked at how to calculate the Rf value of a protein from a western blot. The formula is: Rf = Migration distance of the prote
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