Bestrophin 2 Positive Control peptide (STJ503741)
SPECIFICATIONS
General Information
| Short Description | Bestrophin 2 Positive Control is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Applications | WB |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | WB: 1:500 |
| Formulation | Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Storage Instruction | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | Best2 |
| Gene ID | 212989 |
| Uniprot ID | BEST2_MOUSE |
Additional Info
| Tissue Specificity | Expressed in mucin-secreting colonic goblet cells. |
| Function | Ligand-gated anion channel that allows the movement of anions across cell membranes when activated by calcium (Ca2+). Transports a large specter of anions, namely mediates the movement of chloride, L-glutamate and iodide. Calcium-binding triggers the dilation of the aperture, but calcium-dependent gating is only effective when the size of the passing anion is bigger than the closed aperture. Mediates the calcium-activated hydrogencarbonate movement and participates in colonic hydrogencarbonate secretion concomitant with mucin secretion. In non-pigmented epithelium (NPE), mediates the efflux of intracellular L-glutamate.binding of intracellular L-glutamate activates and open both the neck and the aperture of the channel, leading to L-glutamate exit promoting chloride influx movement from the extracellular side in trans. Also exhibits a directional permeability for intracellular glutamine, in a similar manner as for L-glutamate. |
| Peptide Name | Bestrophin-2Vitelliform Macular Dystrophy 2-Like Protein 1 |
| Database Links | Reactome: R-MMU-2672351 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinBasolateral Cell Membrane |
| Alternative Peptide Names | Bestrophin-2 proteinVitelliform Macular Dystrophy 2-Like Protein 1 proteinBest2 proteinVmd2l1 protein |
Information sourced from Uniprot.org