Atypical Chemokine Receptor D6 Blocking Peptide for STJ500035 peptide (STJ503623)
General Information
| Short Description | Atypical Chemokine Receptor D6 Blocking Peptide for STJ500035 is synthetically produced from the 300-378 sequence and is suitable for use in western blot applications. |
| Applications | Immunodepletion/Immunocompetition |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Formulation | Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice. |
| Storage Instruction | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | Ackr2 |
| Gene ID | 59289 |
| Uniprot ID | ACKR2_MOUSE |
| Immunogen | Synthetic peptide taken within amino acid region 300-378 mouse Atypical chemokine receptor 2 protein. |
| Immunogen Region | 300-378 |
| Specificity | This blocking peptide is recommended for use in combination with Atypical Chemokine Receptor D6 antibody, STJ500035 |
Additional Info
| Tissue Specificity | Expressed on apoptotic neutrophils (at protein level). |
| Post Translational Modifications | Phosphorylated on serine residues in the C-terminal cytoplasmic tail. |
| Function | Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Acts as a receptor for chemokines including CCL2, CCL3, CCL3L1, CCL4, CCL5, CCL7, CCL8, CCL11, CCL13, CCL17, CCL22, CCL23, CCL24, SCYA2/MCP-1, SCY3/MIP-1-alpha, SCYA5/RANTES and SCYA7/MCP-3. Upon active ligand stimulation, activates a beta-arrestin 1 (ARRB1)-dependent, G protein-independent signaling pathway that results in the phosphorylation of the actin-binding protein cofilin (CFL1) through a RAC1-PAK1-LIMK1 signaling pathway. Activation of this pathway results in up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. By scavenging chemokines in tissues, on the surfaces of lymphatic vessels, and in placenta, plays an essential role in the resolution (termination) of the inflammatory response and in the regulation of adaptive immune responses. Plays a major role in the immune silencing of macrophages during the resolution of inflammation. Acts as a regulator of inflammatory leukocyte interactions with lymphatic endothelial cells (LECs) and is required for immature/mature dendritic cells discrimination by LECs. |
| Peptide Name | Atypical Chemokine Receptor 2C-C Chemokine Receptor D6Chemokine-Binding Protein 2Chemokine-Binding Protein D6 |
| Database Links | Reactome: R-MMU-380108 |
| Cellular Localisation | Early EndosomeRecycling EndosomeCell MembraneMulti-Pass Membrane ProteinPredominantly Localizes To Endocytic VesiclesAnd Upon Stimulation By The Ligand Is Internalized Via Clathrin-Coated PitsOnce InternalizedThe Ligand Dissociates From The ReceptorAnd Is Targeted To Degradation While The Receptor Is Recycled Back To The Cell Membrane |
| Alternative Peptide Names | Atypical Chemokine Receptor 2 proteinC-C Chemokine Receptor D6 proteinChemokine-Binding Protein 2 proteinChemokine-Binding Protein D6 proteinAckr2 proteinCcbp2 proteinD6 protein |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Atypical Chemokine Receptor D6 Blocking Peptide for STJ500035 peptide (STJ503623)
This is a blocking peptide intended for use in combination with the Atypical Chemokine Receptor D6 antibody STJ500035. It is used in immunodepletion or immunocompetition experiments to confirm antibody specificity.
This blocking peptide corresponds to a synthetic peptide derived from within amino acid region 300 to 378 of the mouse Atypical Chemokine Receptor 2 protein (UniProt O08707).
This blocking peptide is recommended for immunodepletion and immunocompetition applications, typically to verify that the signal detected by the corresponding antibody is specific to the target antigen.
This blocking peptide is supplied in liquid form at 2.5 mg/ml in PBS, with the option to add up to 5% DMSO. Orders of more than 1 mg can be supplied in lyophilized powder form or in a buffer of choice upon request.
This blocking peptide should be stored at -20 degrees Celsius for long-term storage. Repeated freeze-thaw cycles should be avoided to maintain product integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.