Aromatase Positive Control peptide (STJ505578)

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STJ505578-5

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Applications: WB
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Aromatase Positive Control is synthetically produced from the sequence and is suitable for use in western blot applications.
Formulation: Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is
Dilution Range: WB: 1:2, 500-1:5, 000
Storage Instruction: Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Gene Symbol: CYP19A1
Gene ID: 1588
Uniprot ID: CP19A_HUMAN
Immunogen: Purified Protein
Tissue Specificity Widely expressed, including in adult and fetal brain, placenta, skin fibroblasts, adipose tissue and gonads.
Post Translational Modifications Phosphorylated in vitro by PKA and PKG/PRKG1. These phosphorylations inhibit the catalytic activity as measured by estrone synthesis from androstenedione (36% decrease for PKA and 30% for PKG/PRKG1).
Function A cytochrome P450 monooxygenase that catalyzes the conversion of C19 androgens, androst-4-ene-3,17-dione (androstenedione) and testosterone to the C18 estrogens, estrone and estradiol, respectively. Catalyzes three successive oxidations of C19 androgens: two conventional oxidations at C19 yielding 19-hydroxy and 19-oxo/19-aldehyde derivatives, followed by a third oxidative aromatization step that involves C1-beta hydrogen abstraction combined with cleavage of the C10-C19 bond to yield a phenolic A ring and formic acid. Alternatively, the third oxidative reaction yields a 19-norsteroid and formic acid. Converts dihydrotestosterone to delta1,10-dehydro 19-nordihydrotestosterone and may play a role in homeostasis of this potent androgen. Also displays 2-hydroxylase activity toward estrone. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR.NADPH-ferrihemoprotein reductase).
Peptide Name Aromatase
Cypxix
Cytochrome P-450arom
Cytochrome P450 19a1
Estrogen Synthase
Database Links Reactome: R-HSA-193144
Reactome: R-HSA-211976
Reactome: R-HSA-5579030
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Microsome Membrane
Alternative Peptide Names Aromatase protein
Cypxix protein
Cytochrome P-450arom protein
Cytochrome P450 19a1 protein
Estrogen Synthase protein
CYP19A1 protein
ARO1 protein
CYAR protein
CYP19 protein

Information sourced from Uniprot.org

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