| Short Description : | ARHGEF7 Positive Control for STJ500159 is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Applications: | WB |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Dilution Range: | WB: 1:500 |
| Formulation: | Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | ARHGEF7 |
| Gene ID: | 8874 |
| Uniprot ID: | ARHG7_HUMAN |
| Specificity: | This is positive control is recommended for use in combination with ARHGEF7 antibody STJ500159. |
| Post Translational Modifications | Phosphorylated by PTK2/FAK1.this promotes interaction with RAC1. Phosphorylated on Ser-694 by CaMK1.enhancement of GEF activity and downstream activation of RAC1. |
| Function | Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. Functions in cell migration, attachment and cell spreading. Promotes targeting of RAC1 to focal adhesions. May function as a positive regulator of apoptosis. Downstream of NMDA receptors and CaMKK-CaMK1 signaling cascade, promotes the formation of spines and synapses in hippocampal neurons. |
| Peptide Name | Rho Guanine Nucleotide Exchange Factor 7Beta-PixCool-1Pak-Interacting Exchange Factor BetaP85 |
| Database Links | Reactome: R-HSA-182971Reactome: R-HSA-193648Reactome: R-HSA-3928664Reactome: R-HSA-416482Reactome: R-HSA-8980692Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013406Reactome: R-HSA-9013409Reactome: R-HSA-9013420Reactome: R-HSA-9013424Reactome: R-HSA-9619229 Q14155-1 |
| Cellular Localisation | Cell JunctionFocal AdhesionCell ProjectionRuffleCytoplasmCell CortexLamellipodiumDetected At Cell AdhesionsA Small Proportion Is Detected At Focal Adhesions |
| Alternative Peptide Names | Rho Guanine Nucleotide Exchange Factor 7 proteinBeta-Pix proteinCool-1 proteinPak-Interacting Exchange Factor Beta proteinP85 proteinARHGEF7 proteinCOOL1 proteinKIAA0142 proteinP85SPR proteinPAK3BP proteinPIXB proteinNbla10314 protein |
Information sourced from Uniprot.org
