ARF1 Positive Control for STJ500153 peptide (STJ503700)
SPECIFICATIONS
General Information
| Short Description | ARF1 Positive Control for STJ500153 is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Applications | WB |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | WB: 1:500 |
| Formulation | Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Storage Instruction | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | Arf1 |
| Gene ID | 64310 |
| Uniprot ID | ARF1_RAT |
| Specificity | This is positive control is recommended for use in combination with ARF1 antibody STJ500153. |
Additional Info
| Function | Small GTPase involved in protein trafficking between different compartments. Modulates vesicle budding and uncoating within the Golgi complex. In its GTP-bound form, triggers the recruitment of coatomer proteins to the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity.the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasticity of excitatory synapses and spine shrinkage during long-term depression (LTD). Plays a key role in the regulation of intestinal stem cells and gut microbiota, and is essential for maintaining intestinal homeostasis. Plays also a critical role in mast cell expansion but not in mast cell maturation by facilitating optimal mTORC1 activation. |
| Peptide Name | Adp-Ribosylation Factor 1 |
| Database Links | Reactome: R-RNO-1660499Reactome: -RNO-1660514Reactome: -RNO-199992Reactome: -RNO-2132295Reactome: -RNO-432720Reactome: -RNO-432722Reactome: -RNO-6807878Reactome: -RNO-6811434Reactome: -RNO-6811438Reactome: -RNO-9845576 |
| Cellular Localisation | Golgi Apparatus MembraneLipid-AnchorCytoplasmic SideSynapseSynaptosomePostsynaptic DensityIn The Gdp-Bound FormAssociates Transiently With The Membranes Via Its Myristoylated N-Terminus Where Guanine Nucleotide-Exchange Factor (Gef)-Mediated Nucleotide Exchange OccursFollowing Nucleotide ExchangeThe Gtp-Bound Form Undergoes A Conformational ChangeLeading To The Exposure Of A Myristoylated N-Terminal Amphipathic Helix That Provides Stable Membrane Anchorage |
| Alternative Peptide Names | Adp-Ribosylation Factor 1 proteinArf1 protein |
Information sourced from Uniprot.org