Aquaporin 4 Blocking Peptide is synthetically produced from the 300-323 sequence and is suitable for use in western blot applications.
Applications
Immunodepletion/Immunocompetition
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Formulation
Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice.
Storage Instruction
Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Synthetic peptide taken within amino acid region 300-323 on rat Aquaporin 4 protein.
Immunogen Region
300-323
Additional Info
Tissue Specificity
Detected in skeletal muscle. Detected in stomach, along the glandular base region of the fundic gland (at protein level). Detected in brain, lung and skeletal muscle, and at much lower levels in heart and ovary.
Post Translational Modifications
Phosphorylation by PKC at Ser-180 reduces conductance by 50%. Phosphorylation by PKG at Ser-111 in response to glutamate increases conductance by 40%. Isoform 2: Palmitoylated on its N-terminal region. Isoform 1: Not palmitoylated.
Function
Forms a water-specific channel. Plays an important role in brain water homeostasis. It is involved in glymphatic solute transport and is required for a normal rate of water exchange across the blood brain interface. Required for normal levels of cerebrospinal fluid influx into the brain cortex and parenchyma along paravascular spaces that surround penetrating arteries, and for normal drainage of interstitial fluid along paravenous drainage pathways. Thereby, it is required for normal clearance of solutes from the brain interstitial fluid, including soluble beta-amyloid peptides derived from APP. Plays a redundant role in urinary water homeostasis and urinary concentrating ability.
Peptide Name
Aquaporin-4Aqp-4Mercurial-Insensitive Water ChannelMiwcWch4
Cell MembraneMulti-Pass Membrane ProteinBasolateral Cell MembraneEndosome MembraneSarcolemmaCell ProjectionActivation Of The Vasopressin Receptor Avpr1a Triggers Aqp4 Phosphorylation At Ser-180 And Promotes Its Internalization From The Cell MembraneDetected On Brain Astrocyte Processes And Astrocyte Endfeet Close To Capillaries
Alternative Peptide Names
Aquaporin-4 proteinAqp-4 proteinMercurial-Insensitive Water Channel proteinMiwc proteinWch4 proteinAQP4 protein
