Aquaporin 2 Positive Control for STJ502108 is synthetically produced from the sequence and is suitable for use in western blot applications.
Applications
WB
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Dilution Range
WB: 1:500
Formulation
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is
Storage Instruction
Store at-20°C for long term storage. Avoid freeze-thaw cycles.
This is positive control is recommended for use in combination with Aquaporin 2 antibody STJ502108.
Additional Info
Tissue Specificity
Detected in kidney, in cortical and the medullary collecting tubules (at protein level). Detected in kidney medulla and cortex.
Post Translational Modifications
Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent. N-glycosylated.
Function
Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Plays an essential role in renal water homeostasis. Could also be permeable to glycerol.
Peptide Name
Aquaporin-2Aqp-2Adh Water ChannelAquaporin-CdAqp-CdCollecting Duct Water Channel ProteinWch-CdWater Channel Protein For Renal Collecting Duct
Apical Cell MembraneMulti-Pass Membrane ProteinBasolateral Cell MembraneCell MembraneCytoplasmic Vesicle MembraneGolgi ApparatusTrans-Golgi Network MembraneShuttles From Vesicles To The Apical MembraneVasopressin-Regulated Phosphorylation Is Required For Translocation To The Apical Cell MembranePlekha8/Fapp2 Is Required To Transport Aqp2 From The Tgn To Sites Where Aqp2 Is Phosphorylated
Alternative Peptide Names
Aquaporin-2 proteinAqp-2 proteinAdh Water Channel proteinAquaporin-Cd proteinAqp-Cd proteinCollecting Duct Water Channel Protein proteinWch-Cd proteinWater Channel Protein For Renal Collecting Duct proteinAqp2 protein
