• Immunofluorescence analysis of Raw264. 7 cells using Zyxin Rabbit polyclonal antibody (STJ26156) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using Zyxin Rabbit polyclonal antibody (STJ26156) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Zyxin in paraffin-embedded mouse spleen using Zyxin Rabbit polyclonal antibody (STJ26156) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from HeLa cells, using Zyxin Rabbit polyclonal antibody (STJ26156). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-ZYX antibody (350-572) (STJ26156)

SKU:
STJ26156

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ZYX (350-572) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ZYX
Gene ID: 7791
Uniprot ID: ZYX_HUMAN
Immunogen Region: 350-572
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 350-572 of human Zyxinin (NP_003452.1).
Immunogen Sequence: PLTLKEVEELEQLTQQLMQD MEHPQRQNVAVNELCGRCHQ PLARAQPAVRALGQLFHIAC FTCHQCAQQLQGQQFYSLEG APYCEGCYTDTLEKCNTCGE PITDRMLRATGKAYHPHCFT CVVCARPLEGTSFIVDQANR PHCVPDYHKQYAPRCSVCSE PIMPEPGRDETVRVVALDKN FHMKCYKCEDCGKPLSIEAD DNGCFPLDGHVLCRKCHTAR AQT
Function Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression.
Protein Name Zyxin
Zyxin-2
Cellular Localisation Cytoplasm
Cytoskeleton
Nucleus
Cell Junction
Focal Adhesion
Associates With The Actin Cytoskeleton Near The Adhesion Plaques
Enters The Nucleus In The Presence Of Hesx1
Alternative Antibody Names Anti-Zyxin antibody
Anti-Zyxin-2 antibody
Anti-ZYX antibody

Information sourced from Uniprot.org

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