Anti-ZW10 antibody (38-88 aa) (STJ195391)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human ZW10 at the amino acid range 38-88
General Information
| Short Description | Rabbit polyclonal anti-Centromere/kinetochore protein zw10 homolog (38-88 aa) for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ZW10 |
| Gene ID | 9183 |
| Uniprot ID | ZW10_HUMAN |
| Immunogen | Synthesized peptide derived from the human ZW10 at the amino acid range 38-88 |
| Immunogen Region | 38-88 aa |
| Specificity | This antibody detects endogenous levels of ZW10 at Human/Mouse/Rat |
Additional Info
| Function | Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex. Involved in regulation of membrane traffic between the Golgi and the endoplasmic reticulum (ER).the function is proposed to depend on its association in the interphase NRZ complex which is believed to play a role in SNARE assembly at the ER. |
| Protein Name | Centromere/Kinetochore Protein Zw10 Homolog |
| Database Links | Reactome: R-HSA-141444Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-5663220Reactome: R-HSA-6811434Reactome: R-HSA-68877Reactome: R-HSA-9648025 |
| Cellular Localisation | CytoplasmEndoplasmic Reticulum MembranePeripheral Membrane ProteinChromosomeCentromereKinetochoreCytoskeletonSpindleLipid DropletDynamic Pattern Of Localization During The Cell CycleIn Most Cells At InterphasePresent Diffusely In The CytoplasmIn PrometaphaseAssociated With The KinetochoreAt MetaphaseDetected Both At The Kinetochores AndMost ProminentlyAt The SpindleParticularly At The Spindle PolesIn Very Early AnaphaseDetected On Segregating KinetochoresIn Late Anaphase And TelophaseAccumulates At The Spindle Midzone |
| Alternative Antibody Names | Anti-Centromere/Kinetochore Protein Zw10 Homolog antibodyAnti-ZW10 antibody |
Information sourced from Uniprot.org