Anti-ZP2 antibody (Internal) (STJ96360)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the Internal region of human ZP2.
General Information
| Short Description | Rabbit polyclonal anti-Zona pellucida sperm-binding protein 2 (Internal) for use in IHC, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | IHC 1:100-1:300ELISA 1:40000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ZP2 |
| Gene ID | 7783 |
| Uniprot ID | ZP2_HUMAN |
| Immunogen | Synthesized peptide derived from the Internal region of human ZP2. |
| Immunogen Region | Internal |
| Specificity | ZP2 Polyclonal Antibody detects endogenous levels of ZP2 protein. |
Additional Info
| Post Translational Modifications | Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida. Proteolytically cleaved in the N-terminal part by the metalloendopeptidase ASTL exocytosed from cortical granules after fertilization, yielding a N-terminal peptide of about 30 kDa which remains covalently attached to the C-terminal peptide via disulfide bond(s). This cleavage may play an important role in the post-fertilization block to polyspermy. Additional proteolytically cleavage of the N-terminal peptide of 30 kDa occurs in one-cell and two-cell embryos. N-glycosylated. O-glycosylated.contains sulfate-substituted glycans. |
| Function | Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sperm receptor. |
| Protein Name | Zona Pellucida Sperm-Binding Protein 2Zona Pellucida Glycoprotein 2Zp-2Zona Pellucida Protein A Cleaved Into - Processed Zona Pellucida Sperm-Binding Protein 2 |
| Database Links | Reactome: R-HSA-2534343 |
| Cellular Localisation | Processed Zona Pellucida Sperm-Binding Protein 2: Zona PellucidaCell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-Zona Pellucida Sperm-Binding Protein 2 antibodyAnti-Zona Pellucida Glycoprotein 2 antibodyAnti-Zp-2 antibodyAnti-Zona Pellucida Protein A Cleaved Into - Processed Zona Pellucida Sperm-Binding Protein 2 antibodyAnti-ZP2 antibodyAnti-ZPA antibody |
Information sourced from Uniprot.org