Anti-ZO-1 antibody (Internal) (STJ71854)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-ZO-1 (Internal) is suitable for use in ELISA and Immunofluorescence research applications. |
| Applications | Pep-ELISA/IF |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.IF: Strong expression of the protein seen in the cellular junction of A431 cells, and in the plasma membrane and cytoplasm of U251 cells. Recommended concentration: 10µg/ml. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | TJP1 |
| Gene ID | 7082 |
| Uniprot ID | ZO1_HUMAN |
| Accession Number | NP_003248.3; NP_783297.2 |
| Immunogen Region | Internal |
| Immunogen Sequence | PKPTSQNQFSEHDKT |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_003248.3; NP_783297.2) |
Additional Info
| Post Translational Modifications | Phosphorylated at tyrosine redidues in response to epidermal growth factor (EGF). This response is dependent on an intact actin microfilament system. Dephosphorylated by PTPRJ. |
| Function | TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton. The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells. Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling. With TJP2 and TJP3, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus. May play a role in mediating cell morphology changes during ameloblast differentiation via its role in tight junctions. |
| Protein Name | Tight Junction Protein Zo-1Tight Junction Protein 1Zona Occludens Protein 1Zonula Occludens Protein 1 |
| Database Links | Reactome: R-HSA-191650Reactome: R-HSA-2028269Reactome: R-HSA-351906Reactome: R-HSA-8935964Reactome: R-HSA-9705677 |
| Cellular Localisation | Cell MembranePeripheral Membrane ProteinCytoplasmic SideCell JunctionTight JunctionGap JunctionCell ProjectionPodosomeMoves From The Cytoplasm To The Cell Membrane Concurrently With Cell-Cell ContactAt Podosomal SitesIs Predominantly Localized In The Ring Structure Surrounding The Actin CoreColocalizes With Spef1 At Sites Of Cell-Cell Contact In Intestinal Epithelial Cells |
| Alternative Antibody Names | Anti-Tight Junction Protein Zo-1 antibodyAnti-Tight Junction Protein 1 antibodyAnti-Zona Occludens Protein 1 antibodyAnti-Zonula Occludens Protein 1 antibodyAnti-TJP1 antibodyAnti-ZO1 antibody |
Information sourced from Uniprot.org