• Immunofluorescence analysis of U-2 OS cells using ZNF416 Rabbit polyclonal antibody (STJ117110) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using ZNF416 Rabbit polyclonal antibody (STJ117110) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using ZNF416 Rabbit polyclonal antibody (STJ117110) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of ZNF416 in paraffin-embedded mouse brain using ZNF416 Rabbit polyclonal antibody (STJ117110) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ZNF416 in paraffin-embedded human lung cancer using ZNF416 Rabbit polyclonal antibody (STJ117110) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ZNF416 in paraffin-embedded rat pancreas using ZNF416 Rabbit polyclonal antibody (STJ117110) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using GALNT10 Rabbit polyclonal antibody (A14909) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ZNF416 antibody (495-594) (STJ117110)

SKU:
STJ117110

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Host: Rabbit
Applications: IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ZNF416 (495-594) is suitable for use in Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ZNF416
Gene ID: 55659
Uniprot ID: ZN416_HUMAN
Immunogen Region: 495-594
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 495-594 of human ZNF416 (NP_060349.1).
Immunogen Sequence: ECSECGKFFRQSYTLVEHQK IHTGLRPYDCGQCGKSFIQK SSLIQHQVVHTGERPYECGK CGKSFTQHSGLILHRKSHTV ERPRDSSKCGKPYSPRSNIV
Function May be involved in transcriptional regulation.
Protein Name Zinc Finger Protein 416
Database Links Reactome: R-HSA-212436
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Zinc Finger Protein 416 antibody
Anti-ZNF416 antibody

Information sourced from Uniprot.org

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