Anti-ZNF268 antibody (1-270) (STJ114767)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ZNF268 (1-270) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ZNF268 |
| Gene ID | 10795 |
| Uniprot ID | ZN268_HUMAN |
| Immunogen Region | 1-270 |
| Immunogen Sequence | MATRVRTASIWVPPLQERNS SWDRIRKLQGQESILGQGTP GLQPLPGTPRQKQKSRRIEK VLEWLFISQEQPKITKSWGP LSFMDVFVDFTWEEWQLLDP AQKCLYRSVMLENYSNLVSL GYQHTKPDIIFKLEQGEELC MVQAQVPNQTCPNTVWKIDD LMDWHQENKDKLGSTAKSFE CTTFGKLCLLSTKYLSRQKP HKCGTHGKSLKYIDFTSDYA RNNPNGFQVHGKSFFHSKH |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human ZNF268 (NP_001159353.1). |
Additional Info
| Tissue Specificity | Overexpressed in ovarian cancer tissues compared to normal ovarian tissues. Isoform 1 and isoform 2 are expressed in squamous epithelium tissues. Isoform 2 is overexpressed in squamous cervical cancer (at protein level). Expressed in blood cells. Isoform 1 is expressed in pancreas, lung, skeletal muscle, heart, placenta, liver, kidney and brain. Isoform 2 expressed in chronic lymphocytic leukemia (CLL) and several tumor cell lines. Isoform 3 is expressed in several tumor cells. Isoform 5 is expressed in fetal liver and several tumor cells. Isoform 6 is weakly expressed in brain, lung amd small intestin and in several tumor cells. Isoform 7 is expressed in fetal liver and several tumor cells. |
| Post Translational Modifications | Phosphorylation at Ser-178 stabilizes the protein by interfering with its binding to TOLLIP, hence impairing its degradation by Tollip-mediated selective autophagy system. |
| Function | Isoform 1: Acts as a transcriptional repressor. Inhibits erythroid differentiation and tumor cell proliferation. Plays a role during ovarian cancer development and progression. Isoform 2: Contributes to cervical carcinogenesis in part through the TNF-alpha-induced NF-kappa-B signaling pathway by interacting with the I-kappa-B-kinase (IKK) core complex. Involved in the regulation of antiviral interferon signaling. During viral infection, recruits SETD4 to TBK1, leading to TBK1 monomethylation, which is critical for the assembly of TBK1 complex and IRF3 signaling. |
| Protein Name | Zinc Finger Protein 268Zinc Finger Protein Hzf3 |
| Database Links | Reactome: R-HSA-212436 |
| Cellular Localisation | Isoform 1: NucleusIsoform 2: NucleusCytoplasmUnder Resting ConditionsDegraded By Autophagosomes In The CytosolResulting In Low Expression At The Protein LevelAccumulates In The Cytoplasm During Viral Infection |
| Alternative Antibody Names | Anti-Zinc Finger Protein 268 antibodyAnti-Zinc Finger Protein Hzf3 antibodyAnti-ZNF268 antibody |
Information sourced from Uniprot.org