• RWPE1 lysate (60ug protein in RIPA buffer) overexpressing Human ZIC2 with C-terminal MYC tag probed with STJ72533 (0. 5ug/ml) in the right panel and probed with anti-MYC Tag (1/1000) and anti-beta-Actin in the left panel. Mock-transfected RWPE1 in lanes 1 and expressing GFP in lanes 2. Primary incubations were overnight at 4C. Detected by chemiluminescence.

Anti-ZIC2 antibody (C-Term) (STJ72533)

SKU:
STJ72533-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human/Mouse/Rat/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-ZIC2 (C-Term) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: ELISA-antibody detection limit dilution 1:1000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: ZIC2
Gene ID: 7546
Uniprot ID: ZIC2_HUMAN
Immunogen Region: C-Term
Accession Number: NP_009060.2
Specificity: This antibody is expected to NOT cross-react with other ZIC proteins.
Immunogen Sequence: HSGLSSNFNEWY
Function Acts as a transcriptional activator or repressor. Plays important roles in the early stage of organogenesis of the CNS. Activates the transcription of the serotonin transporter SERT in uncrossed ipsilateral retinal ganglion cells (iRGCs) to refine eye-specific projections in primary visual targets. Its transcriptional activity is repressed by MDFIC. Involved in the formation of the ipsilateral retinal projection at the optic chiasm midline. Drives the expression of EPHB1 on ipsilaterally projecting growth cones. Binds to the minimal GLI-consensus sequence 5'-TGGGTGGTC-3'. Associates to the basal SERT promoter region from ventrotemporal retinal segments of retinal embryos.
Protein Name Zinc Finger Protein Zic 2
Zinc Finger Protein Of The Cerebellum 2
Cellular Localisation Nucleus
Cytoplasm
Localizes In The Cytoplasm In Presence Of Mdfic Overexpression
Both Phosphorylated And Unphosphorylated Forms Are Localized In The Nucleus
Alternative Antibody Names Anti-Zinc Finger Protein Zic 2 antibody
Anti-Zinc Finger Protein Of The Cerebellum 2 antibody
Anti-ZIC2 antibody

Information sourced from Uniprot.org

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