Anti-ZFP36 antibody (STJA0004380)

SKU:
STJA0004380

Current Stock:
Host: Rabbit
Applications: IHC/ELISA
Reactivity: Human/Mouse/Rat
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Mrna Decay Activator Protein Zfp36 is suitable for use in Immunohistochemistry and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
Purification: Antigen affinity purification
Concentration: 0.8 mg/mL
Dilution Range: IHC 1:40-1:200
Storage Instruction: Store at-20°C for long term, and avoid freeze-thaw cycles.
Gene Symbol: ZFP36
Gene ID: 7538
Uniprot ID: TTP_HUMAN
Immunogen: Fusion protein of human ZFP36
Tissue Specificity Expressed in both basal and suprabasal epidermal layers. Expressed in epidermal keratinocytes. Expressed strongly in mature dendritic cells. Expressed in immature dendritic cells (at protein level).
Post Translational Modifications Phosphorylated. Phosphorylation at serine and/or threonine residues occurs in a p38 MAPK- and MAPKAPK2-dependent manner. Phosphorylated by MAPKAPK2 at Ser-60 and Ser-186.phosphorylation increases its stability and cytoplasmic localization, promotes binding to 14-3-3 adapter proteins and inhibits the recruitment of cytoplasmic CCR4-NOT and PAN2-PAN3 deadenylase complexes to the mRNA decay machinery, thereby inhibiting ZFP36-induced ARE-containing mRNA deadenylation and decay processes. Phosphorylation by MAPKAPK2 does not impair ARE-containing RNA-binding. Phosphorylated in a MAPKAPK2- and p38 MAPK-dependent manner upon skeletal muscle satellite cell activation.this phosphorylation inhibits ZFP36-mediated mRNA decay activity, and hence stabilizes MYOD1 mRNA. Phosphorylated by MAPK1 upon mitogen stimulation. Phosphorylated at Ser-66 and Ser-93.these phosphorylations increase in a SH3KBP1-dependent manner. Phosphorylated at serine and threonine residues in a pyruvate kinase PKM- and p38 MAPK-dependent manner. Phosphorylation at Ser-60 may participate in the PKM-mediated degradation of ZFP36 in a p38 MAPK-dependent manner. Dephosphorylated by serine/threonine phosphatase 2A at Ser-186. Ubiquitinated.pyruvate kinase (PKM)-dependent ubiquitination leads to proteasomal degradation through a p38 MAPK signaling pathway.
Function Zinc-finger RNA-binding protein that destabilizes several cytoplasmic AU-rich element (ARE)-containing mRNA transcripts by promoting their poly(A) tail removal or deadenylation, and hence provide a mechanism for attenuating protein synthesis. Acts as an 3'-untranslated region (UTR) ARE mRNA-binding adapter protein to communicate signaling events to the mRNA decay machinery. Recruits deadenylase CNOT7 (and probably the CCR4-NOT complex) via association with CNOT1, and hence promotes ARE-mediated mRNA deadenylation. Functions also by recruiting components of the cytoplasmic RNA decay machinery to the bound ARE-containing mRNAs. Self regulates by destabilizing its own mRNA. Binds to 3'-UTR ARE of numerous mRNAs and of its own mRNA. Plays a role in anti-inflammatory responses.suppresses tumor necrosis factor (TNF)-alpha production by stimulating ARE-mediated TNF-alpha mRNA decay and several other inflammatory ARE-containing mRNAs in interferon (IFN)- and/or lipopolysaccharide (LPS)-induced macrophages. Also plays a role in the regulation of dendritic cell maturation at the post-transcriptional level, and hence operates as part of a negative feedback loop to limit the inflammatory response. Promotes ARE-mediated mRNA decay of hypoxia-inducible factor HIF1A mRNA during the response of endothelial cells to hypoxia. Positively regulates early adipogenesis of preadipocytes by promoting ARE-mediated mRNA decay of immediate early genes (IEGs). Negatively regulates hematopoietic/erythroid cell differentiation by promoting ARE-mediated mRNA decay of the transcription factor STAT5B mRNA. Plays a role in maintaining skeletal muscle satellite cell quiescence by promoting ARE-mediated mRNA decay of the myogenic determination factor MYOD1 mRNA. Associates also with and regulates the expression of non-ARE-containing target mRNAs at the post-transcriptional level, such as MHC class I mRNAs. Participates in association with argonaute RISC catalytic components in the ARE-mediated mRNA decay mechanism.assists microRNA (miRNA) targeting ARE-containing mRNAs. May also play a role in the regulation of cytoplasmic mRNA decapping.enhances decapping of ARE-containing RNAs, in vitro. Involved in the delivery of target ARE-mRNAs to processing bodies (PBs). In addition to its cytosolic mRNA-decay function, affects nuclear pre-mRNA processing. Negatively regulates nuclear poly(A)-binding protein PABPN1-stimulated polyadenylation activity on ARE-containing pre-mRNA during LPS-stimulated macrophages. Also involved in the regulation of stress granule (SG) and P-body (PB) formation and fusion. Plays a role in the regulation of keratinocyte proliferation, differentiation and apoptosis. Plays a role as a tumor suppressor by inhibiting cell proliferation in breast cancer cells. (Microbial infection) Negatively regulates HTLV-1 TAX-dependent transactivation of viral long terminal repeat (LTR) promoter.
Protein Name Mrna Decay Activator Protein Zfp36
G0/G1 Switch Regulatory Protein 24
Growth Factor-Inducible Nuclear Protein Nup475
Tristetraprolin
Zinc Finger Protein 36
Zfp-36
Database Links Reactome: R-HSA-450513
Cellular Localisation Nucleus
Cytoplasm
Cytoplasmic Granule
P-Body
Shuttles Between Nucleus And Cytoplasm In A Crm1-Dependent Manner
Localized Predominantly In The Cytoplasm In A P38 Mapk- And Ywhab-Dependent Manner
Colocalizes With Sh3kbp1 And Map3k4 In The Cytoplasm
Component Of Cytoplasmic Stress Granules (Sgs)
Localizes To Cytoplasmic Stress Granules Upon Energy Starvation
Localizes In Processing Bodies (Pbs)
Excluded From Stress Granules In A Phosphorylation Mapkapk2-Dependent Manner
Shuttles In And Out Of Both Cytoplasmic P-Body And Sgs
(Microbial Infection) Colocalizes With Htlv-1 Tax In The Nucleus And The Cytoplasm In A Region Surrounding The Nucleus
Alternative Antibody Names Anti-Mrna Decay Activator Protein Zfp36 antibody
Anti-G0/G1 Switch Regulatory Protein 24 antibody
Anti-Growth Factor-Inducible Nuclear Protein Nup475 antibody
Anti-Tristetraprolin antibody
Anti-Zinc Finger Protein 36 antibody
Anti-Zfp-36 antibody
Anti-ZFP36 antibody
Anti-G0S24 antibody
Anti-NUP475 antibody
Anti-RNF162A antibody
Anti-TIS11A antibody
Anti-TTP antibody

Information sourced from Uniprot.org

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