• Immunofluorescence analysis of L929 cells using ZC3H7A Rabbit polyclonal antibody (STJ115156) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using ZC3H7A Rabbit polyclonal antibody (STJ115156) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using ZC3H7A Rabbit polyclonal antibody (STJ115156) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using ZC3H7A Rabbit polyclonal antibody (STJ115156) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ZC3H7A antibody (1-220) (STJ115156)

SKU:
STJ115156

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ZC3H7A (1-220) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ZC3H7A
Gene ID: 29066
Uniprot ID: Z3H7A_HUMAN
Immunogen Region: 1-220
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ZC3H7A (NP_054872.2).
Immunogen Sequence: MSNVSEERRKRQQNIKEGLQ FIQSPLSYPGTQEQYAVYLR ALVRNLFNEGNDVYREHDWN NSISQYTEALNIADYAKSEE ILIPKEIIEKLYINRIACYS NMGFHDKVLEDCNIVLSLNA SNCKALYRKSKALSDLGRYK KAYDAVAKCSLAVPQDEHVI KLTQELAQKLGFKIRKAYVR AELSLKSVPGDGATKALNHS VEDIEPDLLTPRQEAVPVVS
Function May be a specific regulator of miRNA biogenesis. Binds to microRNAs MIR7-1, MIR16-2 and MIR29A hairpins recognizing the 3'-ATA(A/T)-5' motif in the apical loop.
Protein Name Zinc Finger Ccch Domain-Containing Protein 7a
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Zinc Finger Ccch Domain-Containing Protein 7a antibody
Anti-ZC3H7A antibody
Anti-ZC3H7 antibody
Anti-ZC3HDC7 antibody
Anti-HSPC055 antibody

Information sourced from Uniprot.org

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