Western blot analysis of extracts of various cell lines, using ZC3H12C antibody (STJ11103365) at 1:1000 dilution. Raw264. 7 cells were treated by LPS (1 Mu g/ml) at 37 ยฐC for 8 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-ZC3H12C antibody (735-883) (STJ11103365)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11103365
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General Information

Short DescriptionRabbit polyclonal ZC3H12C (735-883) antibody for use in WB, IF, ICC and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolZC3H12C
Gene ID85463
Uniprot IDZC12C_HUMAN
Immunogen Region735-883
Immunogen SequenceKAPHLGRSLVATRIDSISDS RLYDSSPSRQRKPYSRQEGL GSWERPGYGIDAYGYRQTYS LPDNSTQPCYEQFTFQSLPE QQEPAWRIPYCGMPQDPPRY QDNREKIYINLCNIFPPDLV RIVMKRNPHMTDAQQLAAAI LVEKSQLGY
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 735-883 of human ZC3H12C (NP_203748.1).

Additional Info

Function May function as RNase and regulate the levels of target RNA species.
Protein Name Probable Ribonuclease Zc3h12c
Mcp-Induced Protein 3
Zinc Finger Ccch Domain-Containing Protein 12c
Database Links
Cellular Localisation
Alternative Antibody Names Anti-Probable Ribonuclease Zc3h12c antibody
Anti-Mcp-Induced Protein 3 antibody
Anti-Zinc Finger Ccch Domain-Containing Protein 12c antibody
Anti-ZC3H12C antibody
Anti-KIAA1726 antibody
Anti-MCPIP3 antibody

Information sourced from Uniprot.org

Citations

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