• Western blot analysis of extracts of various cell lines, using ZBTB17 Antibody (STJ110761) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded mouse heart using ZBTB17 antibody (STJ110761) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human liver damage using ZBTB17 antibody (STJ110761) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded rat brain using ZBTB17 antibody (STJ110761) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human lung cancer using ZBTB17 antibody (STJ110761) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using ZBTB17 antibody (STJ110761) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat brain using ZBTB17 antibody (STJ110761) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human liver damage using ZBTB17 antibody (STJ110761) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse heart using ZBTB17 antibody (STJ110761) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using ZBTB17 Antibody (STJ110761) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ZBTB17 antibody (121-360) (STJ110761)

SKU:
STJ110761

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ZBTB17 (121-360) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ZBTB17
Gene ID: 7709
Uniprot ID: ZBT17_HUMAN
Immunogen Region: 121-360
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 121-360 of human ZBTB17 (NP_003434.2).
Immunogen Sequence: PGGNAEALATEGGDKRAKEE KVATSTLSRLEQAGRSTPIG PSRDLKEERGGQAQSAASGA EQTEKADAPREPPPVELKPD PTSGMAAAEAEAALSESSEQ EMEVEPARKGEEEQKEQEEQ EEEGAGPAEVKEEGSQLENG EAPEENENEESAGTDSGQEL GSEARGLRSGTYGDRTESKA YGSVIHKCEDCGKEFTHTGN FKRHIRIHTGEKPFSCRECS KAFSDPAACKAHEKTHSPL
Tissue Specificity Expressed in germinal center B-cells.
Post Translational Modifications Undergoes 'Lys-48'-linked polyubiquitination at Lys-397 and Lys-481 and subsequent proteasomal degradation in a TRAF2-dependent manner.
Function Transcription factor that can function as an activator or repressor depending on its binding partners, and by targeting negative regulators of cell cycle progression. Plays a critical role in early lymphocyte development, where it is essential to prevent apoptosis in lymphoid precursors, allowing them to survive in response to IL7 and undergo proper lineage commitment. Has been shown to bind to the promoters of adenovirus major late protein and cyclin D1 and activate transcription. Required for early embryonic development during gastrulation. Represses RB1 transcription.this repression can be blocked by interaction with ZBTB49 isoform 3/ZNF509S1.
Protein Name Zinc Finger And Btb Domain-Containing Protein 17
Myc-Interacting Zinc Finger Protein 1
Miz-1
Zinc Finger Protein 151
Zinc Finger Protein 60
Database Links Reactome: R-HSA-381038
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Zinc Finger And Btb Domain-Containing Protein 17 antibody
Anti-Myc-Interacting Zinc Finger Protein 1 antibody
Anti-Miz-1 antibody
Anti-Zinc Finger Protein 151 antibody
Anti-Zinc Finger Protein 60 antibody
Anti-ZBTB17 antibody
Anti-MIZ1 antibody
Anti-ZNF151 antibody
Anti-ZNF60 antibody

Information sourced from Uniprot.org

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