• Immunofluorescence analysis of U-2 OS cells using ZBED1 antibody (STJ28875) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of bewo, using ZBED1 Rabbit polyclonal antibody (STJ28875) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ZBED1 antibody (1-280) (STJ28875)

SKU:
STJ28875

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ZBED1 (1-280) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ZBED1
Gene ID: 9189
Uniprot ID: ZBED1_HUMAN
Immunogen Region: 1-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ZBED1 (NP_001164607.1).
Immunogen Sequence: MENKSLESSQTDLKLVAHPR AKSKVWKYFGFDTNAEGCIL QWKKIYCRICMAQIAYSGNT SNLSYHLEKNHPEEFCEFVK SNTEQMREAFATAFSKLKPE SSQQPGQDALAVKAGHGYDS KKQQELTAAVLGLICEGLYP ASIVDEPTFKVLLKTADPRY ELPSRKYISTKAIPEKYGAV REVILKELAEATWCGISTDM WRSENQNRAYVTLAAHFLGL GAPNCLSMGSRCLKTFEVP
Tissue Specificity Ubiquitously expressed at low levels. Expression is highest in skeletal muscle, heart, spleen and placenta.
Post Translational Modifications Autosumoylated with SUMO1, SUMO2, and SUMO3.
Function Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase which sumoylates CHD3/Mi2-alpha, causing its release from DNA. This results in suppression of CHD3/Mi2-alpha transcription repression, increased recruitment of RNA polymerase II to gene promoters and positive regulation of transcription including H1-5 and ribosomal proteins such as: RPS6, RPL10A, and RPL12. The resulting increased transcriptional activity drives cell proliferation. Binds to 5'-TGTCGCTGACTA-3' consensus sequences in gene promoters of ribosomal proteins. (Microbial infection) Binds to human adenovirus gene promoters and contributes to transcriptional repression and virus growth inhibition during early stages of infection.
Protein Name E3 Sumo-Protein Ligase Zbed1
Dna Replication-Related Element-Binding Factor
Putative Ac-Like Transposable Element
Zinc Finger Bed Domain-Containing Protein 1
Dref Homolog
Database Links Reactome: R-HSA-4551638
Cellular Localisation Nucleus
Pml Body
In Granular Structures
(Microbial Infection) Upon Interaction With Human Adenovirus Early E1a Protein
The Protein Is Redistributed To The Peripheral Areas Of Pml Bodies
Alternative Antibody Names Anti-E3 Sumo-Protein Ligase Zbed1 antibody
Anti-Dna Replication-Related Element-Binding Factor antibody
Anti-Putative Ac-Like Transposable Element antibody
Anti-Zinc Finger Bed Domain-Containing Protein 1 antibody
Anti-Dref Homolog antibody
Anti-ZBED1 antibody
Anti-ALTE antibody
Anti-DREF antibody
Anti-hDREF antibody
Anti-KIAA0785 antibody
Anti-TRAMP antibody

Information sourced from Uniprot.org

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