Anti-YY1 antibody [3F3E7] (STJ16103569)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenA synthetic peptide corresponding to a sequence in the middle region of human YY1, identical to the related mouse sequence.
General Information
| Short Description | Mouse monoclonal anti-YY1 for use in WB, IHC, ICC, IF and FC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/ICC/IF/FC |
| Host | Mouse |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 3F3E7 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Concentration | Adding 0.2 mL of distilled water =f 500 Mu g/mL. |
| Purification | Lyophilized |
| Formulation | Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. NA |
| Storage Instruction | Store at-20°C for up to 1 year. Minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | YY1 |
| Gene ID | 7528 |
| Uniprot ID | TYY1_HUMAN |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human YY1, identical to the related mouse sequence. |
Additional Info
| Function | Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. Binds to the consensus sequence 5'-CCGCCATNTT-3'.some genes have been shown to contain a longer binding motif allowing enhanced binding.the initial CG dinucleotide can be methylated greatly reducing the binding affinity. The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression. For example, it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence. Acts synergistically with the SMAD1 and SMAD4 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions. May play an important role in development and differentiation. Proposed to recruit the PRC2/EED-EZH2 complex to target genes that are transcriptional repressed. Involved in DNA repair. In vitro, binds to DNA recombination intermediate structures (Holliday junctions). Plays a role in regulating enhancer activation. Recruits the PR-DUB complex to specific gene-regulatory regions. Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair.proposed to target the INO80 complex to YY1-responsive elements. |
| Protein Name | Transcriptional Repressor Protein Yy1Delta Transcription FactorIno80 Complex Subunit SNf-E1Yin And Yang 1Yy-1 |
| Database Links | Reactome: R-HSA-5617472Reactome: R-HSA-5689603Reactome: R-HSA-5696394Reactome: R-HSA-8866910Reactome: R-HSA-9018519 |
| Cellular Localisation | Nucleus MatrixAssociated With The Nuclear Matrix |
| Alternative Antibody Names | Anti-Transcriptional Repressor Protein Yy1 antibodyAnti-Delta Transcription Factor antibodyAnti-Ino80 Complex Subunit S antibodyAnti-Nf-E1 antibodyAnti-Yin And Yang 1 antibodyAnti-Yy-1 antibodyAnti-YY1 antibodyAnti-INO80S antibody |
Information sourced from Uniprot.org