| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-YTHDF2 (1-200) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:100-1:500IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
YTHDF2 |
| Gene ID: |
51441 |
| Uniprot ID: |
YTHD2_HUMAN |
| Immunogen Region: |
1-200 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human YTHDF2 (NP_057342.2). |
| Immunogen Sequence: |
MSASSLLEQRPKGQGNKVQN GSVHQKDGLNDDDFEPYLSP QARPNNAYTAMSDSYLPSYY SPSIGFSYSLGEAAWSTGGD TAMPYLTSYGQLSNGEPHFL PDAMFGQPGALGSTPFLGQH GFNFFPSGIDFSAWGNNSSQ GQSTQSSGYSSNYAYAPSSL GGAMIDGQSAFANETLNKAP GMNTIDQGMAALKLGSTEVA |
| Tissue Specificity | Highly expressed in induced pluripotent stem cells (iPSCs) and down-regulated during neural differentiation. |
| Post Translational Modifications | Ubiquitinated by the SCF(SKP2) complex, leading to its degradation. |
| Function | Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates their stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing. Acts as a regulator of mRNA stability by promoting degradation of m6A-containing mRNAs via interaction with the CCR4-NOT and ribonuclease P/MRP complexes, depending on the context. The YTHDF paralogs (YTHDF1, YTHDF2 and YTHDF3) share m6A-containing mRNAs targets and act redundantly to mediate mRNA degradation and cellular differentiation. M6A-containing mRNAs containing a binding site for RIDA/HRSP12 (5'-GGUUC-3') are preferentially degraded by endoribonucleolytic cleavage: cooperative binding of RIDA/HRSP12 and YTHDF2 to transcripts leads to recruitment of the ribonuclease P/MRP complex. Other m6A-containing mRNAs undergo deadenylation via direct interaction between YTHDF2 and CNOT1, leading to recruitment of the CCR4-NOT and subsequent deadenylation of m6A-containing mRNAs. Required maternally to regulate oocyte maturation: probably acts by binding to m6A-containing mRNAs, thereby regulating maternal transcript dosage during oocyte maturation, which is essential for the competence of oocytes to sustain early zygotic development. Also required during spermatogenesis: regulates spermagonial adhesion by promoting degradation of m6A-containing transcripts coding for matrix metallopeptidases. Also involved in hematopoietic stem cells specification by binding to m6A-containing mRNAs, leading to promote their degradation. Also acts as a regulator of neural development by promoting m6A-dependent degradation of neural development-related mRNA targets. Inhibits neural specification of induced pluripotent stem cells by binding to methylated neural-specific mRNAs and promoting their degradation, thereby restraining neural differentiation. Regulates circadian regulation of hepatic lipid metabolism: acts by promoting m6A-dependent degradation of PPARA transcripts. Regulates the innate immune response to infection by inhibiting the type I interferon response: acts by binding to m6A-containing IFNB transcripts and promoting their degradation. May also act as a promoter of cap-independent mRNA translation following heat shock stress: upon stress, relocalizes to the nucleus and specifically binds mRNAs with some m6A methylation mark at their 5'-UTR, protecting demethylation of mRNAs by FTO, thereby promoting cap-independent mRNA translation. Regulates mitotic entry by promoting the phase-specific m6A-dependent degradation of WEE1 transcripts. Promotes formation of phase-separated membraneless compartments, such as P-bodies or stress granules, by undergoing liquid-liquid phase separation upon binding to mRNAs containing multiple m6A-modified residues: polymethylated mRNAs act as a multivalent scaffold for the binding of YTHDF proteins, juxtaposing their disordered regions and thereby leading to phase separation. The resulting mRNA-YTHDF complexes then partition into different endogenous phase-separated membraneless compartments, such as P-bodies, stress granules or neuronal RNA granules. May also recognize and bind RNAs modified by C5-methylcytosine (m5C) and act as a regulator of rRNA processing. (Microbial infection) Promotes viral gene expression and replication of polyomavirus SV40: acts by binding to N6-methyladenosine (m6A)-containing viral RNAs. (Microbial infection) Promotes viral gene expression and virion production of kaposis sarcoma-associated herpesvirus (KSHV) at some stage of the KSHV life cycle (in iSLK.219 and iSLK.BAC16 cells). Acts by binding to N6-methyladenosine (m6A)-containing viral RNAs. |
| Protein Name | Yth Domain-Containing Family Protein 2Df2Cll-Associated Antigen Kw-14High-Glucose-Regulated Protein 8Renal Carcinoma Antigen Ny-Ren-2 |
| Cellular Localisation | CytoplasmCytosolP-BodyStress GranuleNucleusLocalizes To The Cytosol And Relocates To The Nucleus Following Heat Shock StressCan Partition Into Different Structures: Into P-Bodies In Unstressed CellsAnd Into Stress Granules During Stress |
| Alternative Antibody Names | Anti-Yth Domain-Containing Family Protein 2 antibodyAnti-Df2 antibodyAnti-Cll-Associated Antigen Kw-14 antibodyAnti-High-Glucose-Regulated Protein 8 antibodyAnti-Renal Carcinoma Antigen Ny-Ren-2 antibodyAnti-YTHDF2 antibodyAnti-HGRG8 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
