Anti-YTHDF1 antibody (1-50 aa) (STJ92648)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human YTHDF1 at the amino acid range 1-50
General Information
| Short Description | Rabbit polyclonal anti-YTH domain-containing family protein 1 (1-50 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:5000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | YTHDF1 |
| Gene ID | 54915 |
| Uniprot ID | YTHD1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human YTHDF1 at the amino acid range 1-50 |
| Immunogen Region | 1-50 aa |
| Specificity | DACA-1 Polyclonal Antibody detects endogenous levels of DACA-1 protein. |
Additional Info
| Function | Specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and regulates their stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing. Acts as a regulator of mRNA stability by promoting degradation of m6A-containing mRNAs via interaction with the CCR4-NOT complex. The YTHDF paralogs (YTHDF1, YTHDF2 and YTHDF3) shares m6A-containing mRNAs targets and act redundantly to mediate mRNA degradation and cellular differentiation. Required to facilitate learning and memory formation in the hippocampus by binding to m6A-containing neuronal mRNAs. Acts as a regulator of axon guidance by binding to m6A-containing ROBO3 transcripts. Acts as a negative regulator of antigen cross-presentation in myeloid dendritic cells. In the context of tumorigenesis, negative regulation of antigen cross-presentation limits the anti-tumor response by reducing efficiency of tumor-antigen cross-presentation. Promotes formation of phase-separated membraneless compartments, such as P-bodies or stress granules, by undergoing liquid-liquid phase separation upon binding to mRNAs containing multiple m6A-modified residues: polymethylated mRNAs act as a multivalent scaffold for the binding of YTHDF proteins, juxtaposing their disordered regions and thereby leading to phase separation. The resulting mRNA-YTHDF complexes then partition into different endogenous phase-separated membraneless compartments, such as P-bodies, stress granules or neuronal RNA granules. |
| Protein Name | Yth Domain-Containing Family Protein 1Df1Dermatomyositis Associated With Cancer Putative Autoantigen 1Daca-1 |
| Database Links | |
| Cellular Localisation | CytoplasmP-BodyStress Granule |
| Alternative Antibody Names | Anti-Yth Domain-Containing Family Protein 1 antibodyAnti-Df1 antibodyAnti-Dermatomyositis Associated With Cancer Putative Autoantigen 1 antibodyAnti-Daca-1 antibodyAnti-YTHDF1 antibodyAnti-C20orf21 antibody |
Information sourced from Uniprot.org