![Anti-Ythdc1 antibody (597-736) [S5671RM] (STJ11105671)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/178313/370706/STJ11105671_1__59744.1713136645.png?c=1 "Anti-Ythdc1 antibody (597-736) [S5671RM] (STJ11105671)")
![Anti-Ythdc1 antibody (597-736) [S5671RM] (STJ11105671)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/178313/370706/STJ11105671_1__59744.1713136645.png?c=1 "Anti-Ythdc1 antibody (597-736) [S5671RM] (STJ11105671)")
Anti-Ythdc1 antibody (597-736) [S5671RM] (STJ11105671)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal Ythdc1 (597-736) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S5671RM |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | Ythdc1 |
| Gene ID | 231386 |
| Uniprot ID | YTDC1_MOUSE |
| Immunogen Region | 597-736 |
| Immunogen Sequence | SYNDYVREFHNMGPPPPWQG MPPYPGIEQPPHHPYYQHHA PPPQAHPPYSGHHPVPHEAR YRDKRVHDYDMRVDDFLRRT QAVVSGRRSRPRERDRERER DRPRDNRRDRERDRGRDRER ERERICDRDRDRGERGRYRR |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 597-736 of mouse Ythdc1 (NP_808348.2). |
Additional Info
| Post Translational Modifications | Tyrosine phosphorylated. |
| Function | Regulator of alternative splicing that specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. Acts as a key regulator of exon-inclusion or exon-skipping during alternative splicing via interaction with mRNA splicing factors SRSF3 and SRSF10. Specifically binds m6A-containing mRNAs and promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing. In contrast, interaction with SRSF3 prevents interaction with SRSF10, a splicing factor that promotes exon skipping: this prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing. May also regulate alternative splice site selection. Also involved in nuclear export of m6A-containing mRNAs via interaction with SRSF3: interaction with SRSF3 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export. Involved in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts, probably by binding m6A-containing MAT2A mRNAs. Also recognizes and binds m6A on other RNA molecules. Involved in random X inactivation mediated by Xist RNA: recognizes and binds m6A-containing Xist and promotes transcription repression activity of Xist. Also recognizes and binds m6A-containing single-stranded DNA. Involved in germline development: required for spermatogonial development in males and oocyte growth and maturation in females, probably via its role in alternative splicing. |
| Protein Name | Yth Domain-Containing Protein 1 |
| Database Links | |
| Cellular Localisation | NucleusNucleus SpeckleLocalizes To A Novel Subnuclear StructureThe Yt Bodies |
| Alternative Antibody Names | Anti-Yth Domain-Containing Protein 1 antibodyAnti-Ythdc1 antibodyAnti-Kiaa1966 antibody |
Information sourced from Uniprot.org