Anti-YARS antibody (1-300) (STJ28874)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal YARS (1-300) antibody for use in WB, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | YARS1 |
| Gene ID | 8565 |
| Uniprot ID | SYYC_HUMAN |
| Immunogen Region | 1-300 |
| Immunogen Sequence | MGDAPSPEEKLHLITRNLQE VLGEEKLKEILKERELKIYW GTATTGKPHVAYFVPMSKIA DFLKAGCEVTILFADLHAYL DNMKAPWELLELRVSYYENV IKAMLESIGVPLEKLKFIKG TDYQLSKEYTLDVYRLSSVV TQHDSKKAGAEVVKQVEHPL LSGLLYPGLQALDEEYLKVD AQFGGIDQRKIFTFAEKYLP ALGYSKRVHLMNPMVPGLTG SKMSSSEEESKIDLLDRKE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human YARS (NP_003671.1). |
Additional Info
| Function | Tyrosine--tRNA ligase that catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) (Probable). Also acts as a positive regulator of poly-ADP-ribosylation in the nucleus, independently of its tyrosine--tRNA ligase activity. Activity is switched upon resveratrol-binding: resveratrol strongly inhibits the tyrosine--tRNA ligase activity and promotes relocalization to the nucleus, where YARS1 specifically stimulates the poly-ADP-ribosyltransferase activity of PARP1. |
| Protein Name | Tyrosine--Trna Ligase - CytoplasmicTyrosyl-Trna SynthetaseTyrrs Cleaved Into - Tyrosine--Trna Ligase - Cytoplasmic - N-Terminally Processed |
| Database Links | Reactome: R-HSA-379716 |
| Cellular Localisation | CytoplasmNucleusCytoplasmic In Normal ConditionsResveratrol-Binding In Response To Serum Starvation Promotes Relocalization To The Nucleus |
| Alternative Antibody Names | Anti-Tyrosine--Trna Ligase - Cytoplasmic antibodyAnti-Tyrosyl-Trna Synthetase antibodyAnti-Tyrrs Cleaved Into - Tyrosine--Trna Ligase - Cytoplasmic - N-Terminally Processed antibodyAnti-YARS1 antibodyAnti-YARS antibody |
Information sourced from Uniprot.org