| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-Y14/RBM8A (1-100) is suitable for use in Western Blot research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S9MR |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RBM8A |
| Gene ID: |
9939 |
| Uniprot ID: |
RBM8A_HUMAN |
| Immunogen Region: |
1-100 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Y14/RBM8A (Q9Y5S9). |
| Immunogen Sequence: |
MADVLDLHEAGGEDFAMDED GDESIHKLKEKAKKRKGRGF GSEEGSRARMREDYDSVEQD GDEPGPQRSVEGWILFVTGV HEEATEEDIHDKFAEYGEIK |
| Tissue Specificity | Ubiquitous. |
| Function | Required for pre-mRNA splicing as component of the spliceosome. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator PYM1 leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Its removal from cytoplasmic mRNAs requires translation initiation from EJC-bearing spliced mRNAs. Associates preferentially with mRNAs produced by splicing. Does not interact with pre-mRNAs, introns, or mRNAs produced from intronless cDNAs. Associates with both nuclear mRNAs and newly exported cytoplasmic mRNAs. The MAGOH-RBM8A heterodimer is a component of the nonsense mediated decay (NMD) pathway. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes).specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S).the function is different from the established EJC assembly. |
| Protein Name | Rna-Binding Protein 8aBinder Of Ovca1-1Bov-1Rna-Binding Motif Protein 8aRna-Binding Protein Y14Ribonucleoprotein Rbm8a |
| Database Links | Reactome: R-HSA-159236Reactome: R-HSA-72163Reactome: R-HSA-72187Reactome: R-HSA-73856Reactome: R-HSA-9010553Reactome: R-HSA-975957 |
| Cellular Localisation | NucleusNucleus SpeckleCytoplasmNucleocytoplasmic Shuttling ProteinTravels To The Cytoplasm As Part Of The Exon Junction Complex (Ejc) Bound To MrnaColocalizes With The Core EjcAlyref/Thoc4Nxf1 And Uap56 In The Nucleus And Nuclear Speckles |
| Alternative Antibody Names | Anti-Rna-Binding Protein 8a antibodyAnti-Binder Of Ovca1-1 antibodyAnti-Bov-1 antibodyAnti-Rna-Binding Motif Protein 8a antibodyAnti-Rna-Binding Protein Y14 antibodyAnti-Ribonucleoprotein Rbm8a antibodyAnti-RBM8A antibodyAnti-RBM8 antibodyAnti-HSPC114 antibodyAnti-MDS014 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
