• Western blot analysis of lysates from LOVO cells, using Ku70/80 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Ku70/80 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Immunofluorescence analysis of A549 cells, using Ku70/80 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of COS7 cells using Ku-80 Polyclonal Antibody
  • Western blot analysis of various cells using Ku-80 Polyclonal Antibody
  • Western blot analysis of lysates from 1) K562, 2) Jurkat, 3) COS7 cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. (Red) GAPDH monoclonal antibody (2B8) (cat: (STJ96931) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-XRCC5 antibody (683-732 aa) (STJ93866)

SKU:
STJ93866

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-X-ray repair cross-complementing protein 5 (683-732 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: XRCC5
Gene ID: 7520
Uniprot ID: XRCC5_HUMAN
Immunogen Region: 683-732 aa
Specificity: Ku-80 Polyclonal Antibody detects endogenous levels of Ku-80 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Ku70/80 at the amino acid range 683-732
Function Single-stranded DNA-dependent ATP-dependent helicase that plays a key role in DNA non-homologous end joining (NHEJ) by recruiting DNA-PK to DNA. Required for double-strand break repair and V(D)J recombination. Also has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. During NHEJ, the XRCC5-XRRC6 dimer performs the recognition step: it recognizes and binds to the broken ends of the DNA and protects them from further resection. Binding to DNA may be mediated by XRCC6. The XRCC5-XRRC6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5-XRRC6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. The XRCC5-XRRC6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5-XRRC6 dimer together with APEX1 acts as a negative regulator of transcription. In association with NAA15, the XRCC5-XRRC6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. As part of the DNA-PK complex, involved in the early steps of ribosome assembly by promoting the processing of precursor rRNA into mature 18S rRNA in the small-subunit processome. Binding to U3 small nucleolar RNA, recruits PRKDC and XRCC5/Ku86 to the small-subunit processome. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.
Protein Name X-Ray Repair Cross-Complementing Protein 5
86 Kda Subunit Of Ku Antigen
Atp-Dependent Dna Helicase 2 Subunit 2
Atp-Dependent Dna Helicase Ii 80 Kda Subunit
Ctc Box-Binding Factor 85 Kda Subunit
Ctc85
Ctcbf
Dna Repair Protein Xrcc5
Ku80
Ku86
Lupus Ku Autoantigen Protein P86
Nuclear Factor Iv
Thyroid-Lupus Autoantigen
Tlaa
X-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 5
Double-Strand-Break Rejoining
Database Links Reactome: R-HSA-164843
Reactome: R-HSA-1834949
Reactome: R-HSA-3270619
Reactome: R-HSA-5693571
Reactome: R-HSA-6798695
Cellular Localisation Nucleus
Nucleolus
Chromosome
Alternative Antibody Names Anti-X-Ray Repair Cross-Complementing Protein 5 antibody
Anti-86 Kda Subunit Of Ku Antigen antibody
Anti-Atp-Dependent Dna Helicase 2 Subunit 2 antibody
Anti-Atp-Dependent Dna Helicase Ii 80 Kda Subunit antibody
Anti-Ctc Box-Binding Factor 85 Kda Subunit antibody
Anti-Ctc85 antibody
Anti-Ctcbf antibody
Anti-Dna Repair Protein Xrcc5 antibody
Anti-Ku80 antibody
Anti-Ku86 antibody
Anti-Lupus Ku Autoantigen Protein P86 antibody
Anti-Nuclear Factor Iv antibody
Anti-Thyroid-Lupus Autoantigen antibody
Anti-Tlaa antibody
Anti-X-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 5 antibody
Anti-Double-Strand-Break Rejoining antibody
Anti-XRCC5 antibody
Anti-G22P2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance