• Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g Ku80 antibody (STJ11101409). Western blot was performed from the immunoprecipitate using Ku80 antibody (STJ11101409) at a dilution of 1:1000.
  • Confocal imaging of HeLa cells using Ku80 Rabbit monoclonal antibody (STJ11101409, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.
  • Immunohistochemistry analysis of Ku80 in paraffin-embedded human esophageal using Ku80 Rabbit monoclonal antibody (STJ11101409) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using Ku80 Rabbit monoclonal antibody (STJ11101409) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-XRCC5 antibody (633-732) [S9MR] (STJ11101409)

SKU:
STJ11101409

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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Ku80 (633-732) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: XRCC5
Gene ID: 7520
Uniprot ID: XRCC5_HUMAN
Immunogen Region: 633-732
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 633-732 of human Ku80 (P13010).
Immunogen Sequence: MKSIDCIRAFREEAIKFSEE QRFNNFLKALQEKVEIKQLN HFWEIVVQDGITLITKEEAS GSSVTAEEAKKFLAPKDKPS GDTAAVFEEGGDVDDLLDMI
Post Translational Modifications ADP-ribosylated by PARP3. Phosphorylated on serine residues. Phosphorylation by PRKDC may enhance helicase activity. Sumoylated. Ubiquitinated by RNF8 via 'Lys-48'-linked ubiquitination following DNA damage, leading to its degradation and removal from DNA damage sites. Ubiquitinated by RNF138, leading to remove the Ku complex from DNA breaks.
Function Single-stranded DNA-dependent ATP-dependent helicase that plays a key role in DNA non-homologous end joining (NHEJ) by recruiting DNA-PK to DNA. Required for double-strand break repair and V(D)J recombination. Also has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. During NHEJ, the XRCC5-XRRC6 dimer performs the recognition step: it recognizes and binds to the broken ends of the DNA and protects them from further resection. Binding to DNA may be mediated by XRCC6. The XRCC5-XRRC6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5-XRRC6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. The XRCC5-XRRC6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5-XRRC6 dimer together with APEX1 acts as a negative regulator of transcription. In association with NAA15, the XRCC5-XRRC6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. As part of the DNA-PK complex, involved in the early steps of ribosome assembly by promoting the processing of precursor rRNA into mature 18S rRNA in the small-subunit processome. Binding to U3 small nucleolar RNA, recruits PRKDC and XRCC5/Ku86 to the small-subunit processome. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.
Protein Name X-Ray Repair Cross-Complementing Protein 5
86 Kda Subunit Of Ku Antigen
Atp-Dependent Dna Helicase 2 Subunit 2
Atp-Dependent Dna Helicase Ii 80 Kda Subunit
Ctc Box-Binding Factor 85 Kda Subunit
Ctc85
Ctcbf
Dna Repair Protein Xrcc5
Ku80
Ku86
Lupus Ku Autoantigen Protein P86
Nuclear Factor Iv
Thyroid-Lupus Autoantigen
Tlaa
X-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 5
Double-Strand-Break Rejoining
Database Links Reactome: R-HSA-164843
Reactome: R-HSA-1834949
Reactome: R-HSA-3270619
Reactome: R-HSA-5693571
Reactome: R-HSA-6798695
Cellular Localisation Nucleus
Nucleolus
Chromosome
Alternative Antibody Names Anti-X-Ray Repair Cross-Complementing Protein 5 antibody
Anti-86 Kda Subunit Of Ku Antigen antibody
Anti-Atp-Dependent Dna Helicase 2 Subunit 2 antibody
Anti-Atp-Dependent Dna Helicase Ii 80 Kda Subunit antibody
Anti-Ctc Box-Binding Factor 85 Kda Subunit antibody
Anti-Ctc85 antibody
Anti-Ctcbf antibody
Anti-Dna Repair Protein Xrcc5 antibody
Anti-Ku80 antibody
Anti-Ku86 antibody
Anti-Lupus Ku Autoantigen Protein P86 antibody
Anti-Nuclear Factor Iv antibody
Anti-Thyroid-Lupus Autoantigen antibody
Anti-Tlaa antibody
Anti-X-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 5 antibody
Anti-Double-Strand-Break Rejoining antibody
Anti-XRCC5 antibody
Anti-G22P2 antibody

Information sourced from Uniprot.org

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