Anti-XRCC4-like factor/NHEJ1 antibody (C-Term) (STJ71008)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-XRCC4-like factor/NHEJ1 (C-Term) is suitable for use in ELISA, Western Blot, Immunofluorescence, Immunohistochemistry and Flow Cytometry research applications. |
| Applications | Pep-ELISA/WB/IF/IHC/FC |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx. 38kDa band observed in nuclear lysates of cell lines HeLa, A431, Jurkat and K562, and approx. 37kDa in Human Skeletal muscle and in nuclear lysates of cell line HepG2 (calculat |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | NHEJ1 |
| Gene ID | 79840 |
| Uniprot ID | NHEJ1_HUMAN |
| Accession Number | NP_079058.1 |
| Immunogen Region | C-Term |
| Immunogen Sequence | QRPQLSKVKRKKPR |
Additional Info
| Post Translational Modifications | Phosphorylated by PRKDC at the C-terminus in response to DNA damage. Phosphorylations by PRKDC at the C-terminus of XRCC4 and NHEJ1/XLF are highly redundant and regulate ability of the XRCC4-NHEJ1/XLF subcomplex to bridge DNA. Phosphorylation does not prevent interaction with XRCC4 but disrupts ability to bridge DNA and promotes detachment from DNA. |
| Function | DNA repair protein involved in DNA non-homologous end joining (NHEJ).required for double-strand break (DSB) repair and V(D)J recombination. Plays a key role in NHEJ by promoting the ligation of various mismatched and non-cohesive ends. Together with PAXX, collaborates with DNA polymerase lambda (POLL) to promote joining of non-cohesive DNA ends. May act in concert with XRCC5-XRCC6 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are non-complementary or partially complementary. In some studies, has been shown to associate with XRCC4 to form alternating helical filaments that bridge DNA and act like a bandage, holding together the broken DNA until it is repaired. Alternatively, it has also been shown that rather than forming filaments, a single NHEJ1 dimer interacts through both head domains with XRCC4 to promote the close alignment of DNA ends. The XRCC4-NHEJ1/XLF subcomplex binds to the DNA fragments of a DSB in a highly diffusive manner and robustly bridges two independent DNA molecules, holding the broken DNA fragments in close proximity to one other. The mobility of the bridges ensures that the ends remain accessible for further processing by other repair factors. Binds DNA in a length-dependent manner. |
| Protein Name | Non-Homologous End-Joining Factor 1Protein CernunnosXrcc4-Like Factor |
| Database Links | Reactome: R-HSA-5693571 |
| Cellular Localisation | NucleusChromosomeLocalizes To Site Of Double-Strand BreaksRecruitment Is Dependent On Xrcc5-Xrcc6 (Ku) Heterodimer |
| Alternative Antibody Names | Anti-Non-Homologous End-Joining Factor 1 antibodyAnti-Protein Cernunnos antibodyAnti-Xrcc4-Like Factor antibodyAnti-NHEJ1 antibodyAnti-XLF antibody |
Information sourced from Uniprot.org