Anti-XRCC1 antibody [R05-7K6] (STJA0036386)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human XRCC1
General Information
| Short Description | Rabbit monoclonal anti-XRCC1 for use in WB, IHC-P, ICC and IF in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ICC/IF |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R05-7K6 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Chromatography |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200 |
| Formulation | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | XRCC1 |
| Gene ID | 7515 |
| Uniprot ID | XRCC1_HUMAN |
| Immunogen | A synthesized peptide derived from human XRCC1 |
Additional Info
| Tissue Specificity | Expressed in fibroblasts, retinal pigmented epithelial cells and lymphoblastoid cells (at protein level). |
| Post Translational Modifications | Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF. Sumoylated. |
| Function | Scaffold protein involved in DNA single-strand break repair by mediating the assembly of DNA break repair protein complexes. Negatively regulates ADP-ribosyltransferase activity of PARP1 during base-excision repair in order to prevent excessive PARP1 activity. Recognizes and binds poly-ADP-ribose chains: specifically binds auto-poly-ADP-ribosylated PARP1, limiting its activity. |
| Protein Name | Dna Repair Protein Xrcc1X-Ray Repair Cross-Complementing Protein 1 |
| Database Links | Reactome: R-HSA-110381Reactome: R-HSA-5649702Reactome: R-HSA-5685939Reactome: R-HSA-5696397Reactome: R-HSA-6782210 |
| Cellular Localisation | NucleusChromosomeMoves From The Nucleoli To The Global Nuclear Chromatin Upon Dna DamageRecruited To Dna Damage Sites Fowwing Interaction With Poly-Adp-Ribose Chains |
| Alternative Antibody Names | Anti-Dna Repair Protein Xrcc1 antibodyAnti-X-Ray Repair Cross-Complementing Protein 1 antibodyAnti-XRCC1 antibody |
Information sourced from Uniprot.org