• Immunofluorescence analysis of HeLa cells using XRCC1 antibody (STJ26128) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse lung using XRCC1 Rabbit polyclonal antibody (STJ26128) at dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using XRCC1 Rabbit polyclonal antibody (STJ26128) at dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using XRCC1 antibody (STJ26128) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-XRCC1 antibody (480-633) (STJ26128)

SKU:
STJ26128

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-XRCC1 (480-633) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: XRCC1
Gene ID: 7515
Uniprot ID: XRCC1_HUMAN
Immunogen Region: 480-633
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 480-633 of human XRCC1 (NP_006288.2).
Immunogen Sequence: NGAEDSGDTEDELRRVAEQK EHRLPPGQEENGEDPYAGST DENTDSEEHQEPPDLPVPEL PDFFQGKHFFLYGEFPGDER RKLIRYVTAFNGELEDYMSD RVQFVITAQEWDPSFEEALM DNPSLAFVRPRWIYSCNEKQ KLLPHQLYGVVPQA
Tissue Specificity Expressed in fibroblasts, retinal pigmented epithelial cells and lymphoblastoid cells (at protein level).
Post Translational Modifications Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF. Sumoylated.
Function Scaffold protein involved in DNA single-strand break repair by mediating the assembly of DNA break repair protein complexes. Negatively regulates ADP-ribosyltransferase activity of PARP1 during base-excision repair in order to prevent excessive PARP1 activity. Recognizes and binds poly-ADP-ribose chains: specifically binds auto-poly-ADP-ribosylated PARP1, limiting its activity.
Protein Name Dna Repair Protein Xrcc1
X-Ray Repair Cross-Complementing Protein 1
Database Links Reactome: R-HSA-110381
Reactome: R-HSA-5649702
Reactome: R-HSA-5685939
Reactome: R-HSA-5696397
Reactome: R-HSA-6782210
Cellular Localisation Nucleus
Chromosome
Moves From The Nucleoli To The Global Nuclear Chromatin Upon Dna Damage
Recruited To Dna Damage Sites Fowwing Interaction With Poly-Adp-Ribose Chains
Alternative Antibody Names Anti-Dna Repair Protein Xrcc1 antibody
Anti-X-Ray Repair Cross-Complementing Protein 1 antibody
Anti-XRCC1 antibody

Information sourced from Uniprot.org

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