Anti-XRCC1 antibody (480-633) (STJ26128)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal XRCC1 (480-633) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:100-1:500IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | XRCC1 |
| Gene ID | 7515 |
| Uniprot ID | XRCC1_HUMAN |
| Immunogen Region | 480-633 |
| Immunogen Sequence | NGAEDSGDTEDELRRVAEQK EHRLPPGQEENGEDPYAGST DENTDSEEHQEPPDLPVPEL PDFFQGKHFFLYGEFPGDER RKLIRYVTAFNGELEDYMSD RVQFVITAQEWDPSFEEALM DNPSLAFVRPRWIYSCNEKQ KLLPHQLYGVVPQA |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 480-633 of human XRCC1 (NP_006288.2). |
Additional Info
| Tissue Specificity | Expressed in fibroblasts, retinal pigmented epithelial cells and lymphoblastoid cells (at protein level). |
| Post Translational Modifications | Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF. Sumoylated. |
| Function | Scaffold protein involved in DNA single-strand break repair by mediating the assembly of DNA break repair protein complexes. Negatively regulates ADP-ribosyltransferase activity of PARP1 during base-excision repair in order to prevent excessive PARP1 activity. Recognizes and binds poly-ADP-ribose chains: specifically binds auto-poly-ADP-ribosylated PARP1, limiting its activity. |
| Protein Name | Dna Repair Protein Xrcc1X-Ray Repair Cross-Complementing Protein 1 |
| Database Links | Reactome: R-HSA-110381Reactome: R-HSA-5649702Reactome: R-HSA-5685939Reactome: R-HSA-5696397Reactome: R-HSA-6782210 |
| Cellular Localisation | NucleusChromosomeMoves From The Nucleoli To The Global Nuclear Chromatin Upon Dna DamageRecruited To Dna Damage Sites Fowwing Interaction With Poly-Adp-Ribose Chains |
| Alternative Antibody Names | Anti-Dna Repair Protein Xrcc1 antibodyAnti-X-Ray Repair Cross-Complementing Protein 1 antibodyAnti-XRCC1 antibody |
Information sourced from Uniprot.org