• Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Exportin 5 (XPO5) (XPO5) Rabbit monoclonal antibody (STJ11101954) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Exportin 5 (XPO5) (XPO5) Rabbit monoclonal antibody (STJ11101954) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using Exportin 5 (XPO5) (XPO5) Rabbit monoclonal antibody (STJ11101954) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-XPO5 antibody (1-200) [S4MR] (STJ11101954)

SKU:
STJ11101954

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Exportin 5 (1-200) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000 IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: XPO5
Gene ID: 57510
Uniprot ID: XPO5_HUMAN
Immunogen Region: 1-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human Exportin 5 (XPO5) (Q9HAV4).
Immunogen Sequence: MAMDQVNALCEQLVKAVTVM MDPNSTQRYRLEALKFCEEF KEKCPICVPCGLRLAEKTQV AIVRHFGLQILEHVVKFRWN GMSRLEKVYLKNSVMELIAN GTLNILEEENHIKDALSRIV VEMIKREWPQHWPDMLIELD TLSKQGETQTELVMFILLRL AEDVVTFQTLPPQRRRDIQQ TLTQNMERIFSFLLNTLQEN
Tissue Specificity Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas.
Function Mediates the nuclear export of proteins bearing a double-stranded RNA binding domain (dsRBD) and double-stranded RNAs (cargos). XPO5 in the nucleus binds cooperatively to the RNA and to the GTPase Ran in its active GTP-bound form. Proteins containing dsRBDs can associate with this trimeric complex through the RNA. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause disassembly of the complex and release of the cargo from the export receptor. XPO5 then returns to the nuclear compartment by diffusion through the nuclear pore complex, to mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Overexpression may in some circumstances enhance RNA-mediated gene silencing (RNAi). Mediates nuclear export of isoform 5 of ADAR/ADAR1 in a RanGTP-dependent manner. Mediates the nuclear export of micro-RNA precursors, which form short hairpins. Also mediates the nuclear export of synthetic short hairpin RNAs used for RNA interference. In some circumstances can also mediate the nuclear export of deacylated and aminoacylated tRNAs. Specifically recognizes dsRNAs that lack a 5'-overhang in a sequence-independent manner, have only a short 3'-overhang, and that have a double-stranded length of at least 15 base-pairs. Binding is dependent on Ran-GTP. (Microbial infection) Mediates the nuclear export of adenovirus VA1 dsRNA.
Protein Name Exportin-5
Exp5
Ran-Binding Protein 21
Database Links Reactome: R-HSA-203927
Cellular Localisation Nucleus
Cytoplasm
Shuttles Between The Nucleus And The Cytoplasm
Alternative Antibody Names Anti-Exportin-5 antibody
Anti-Exp5 antibody
Anti-Ran-Binding Protein 21 antibody
Anti-XPO5 antibody
Anti-KIAA1291 antibody
Anti-RANBP21 antibody

Information sourced from Uniprot.org

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