• Immunofluorescence analysis of L929 cells using XPNPEP3 Rabbit polyclonal antibody (STJ117118) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using XPNPEP3 Rabbit polyclonal antibody (STJ117118) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunofluorescence analysis of U-2 OS cells using KLHL8 Rabbit polyclonal antibody (A14917) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using KLHL8 Rabbit polyclonal antibody (A14917) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using KLHL8 Rabbit polyclonal antibody (A14917) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using KLHL8 Rabbit polyclonal antibody (A14917) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-XPNPEP3 antibody (1-250) (STJ117118)

SKU:
STJ117118

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-XPNPEP3 (1-250) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: XPNPEP3
Gene ID: 63929
Uniprot ID: XPP3_HUMAN
Immunogen Region: 1-250
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human XPNPEP3 (NP_071381.1).
Immunogen Sequence: MPWLLSAPKLVPAVANVRGL SGCMLCSQRRYSLQPVPERR IPNRYLGQPSPFTHPHLLRP GEVTPGLSQVEYALRRHKLM SLIQKEAQGQSGTDQTVVVL SNPTYYMSNDIPYTFHQDNN FLYLCGFQEPDSILVLQSLP GKQLPSHKAILFVPRRDPSR ELWDGPRSGTDGAIALTGVD EAYTLEEFQHLLPKMKAETN MVWYDWMRPSHAQLHSDYMQ PLTEAKAKSKNKVRGVQQL
Tissue Specificity Isoform 1 and isoform 2 are widely expressed, with isoform 1 being more abundant.
Function Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Leu-Pro-Ala. Also shows low activity towards peptides with Ala or Ser at the P1 position. Isoform 1: Promotes TNFRSF1B-mediated phosphorylation of MAPK8/JNK1 and MAPK9/JNK2, suggesting a function as an adapter protein for TNFRSF1B.the effect is independent of XPNPEP3 peptidase activity. May inhibit apoptotic cell death induced via TNF-TNFRSF1B signaling.
Protein Name Xaa-Pro Aminopeptidase 3
X-Pro Aminopeptidase 3
Aminopeptidase P3
App3
Cellular Localisation Isoform 1: Mitochondrion
Cytoplasm
Mainly Mitochondrial
Translocates To The Cytoplasm Following Tnfrsf1b Activation
Isoform 2: Cytoplasm
Alternative Antibody Names Anti-Xaa-Pro Aminopeptidase 3 antibody
Anti-X-Pro Aminopeptidase 3 antibody
Anti-Aminopeptidase P3 antibody
Anti-App3 antibody
Anti-XPNPEP3 antibody

Information sourced from Uniprot.org

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