• Western blot analysis of lysates from wild type (WT) and XIAP knockout (KO) 293T cells, using [KO Validated] XIAP Rabbit monoclonal antibody (STJ11104809) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of various lysates using [KO Validated] XIAP Rabbit monoclonal antibody (STJ11104809) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-XIAP antibody [S4809RM] (STJ11104809)

SKU:
STJ11104809

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-E3 Ubiquitin-Protein Ligase Xiap is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4809RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:6000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: XIAP
Gene ID: 331
Uniprot ID: XIAP_HUMAN
Immunogen: Recombinant protein of human XIAP.
Tissue Specificity Expressed in colonic crypts (at protein level). Ubiquitous, except peripheral blood leukocytes.
Post Translational Modifications S-Nitrosylation down-regulates its E3 ubiquitin-protein ligase activity. Autoubiquitinated.
Function Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, copper homeostasis, mitogenic kinase signaling, cell proliferation, as well as cell invasion and metastasis. Acts as a direct caspase inhibitor. Directly bind to the active site pocket of CASP3 and CASP7 and obstructs substrate entry. Inactivates CASP9 by keeping it in a monomeric, inactive state. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and the target proteins for its E3 ubiquitin-protein ligase activity include: RIPK1, RIPK2, MAP3K2/MEKK2, DIABLO/SMAC, AIFM1, CCS, PTEN and BIRC5/survivin. Acts as an important regulator of innate immunity by mediating 'Lys-63'-linked polyubiquitination of RIPK2 downstream of NOD1 and NOD2, thereby transforming RIPK2 into a scaffolding protein for downstream effectors, ultimately leading to activation of the NF-kappa-B and MAP kinases signaling. 'Lys-63'-linked polyubiquitination of RIPK2 also promotes recruitment of the LUBAC complex to RIPK2. Regulates the BMP signaling pathway and the SMAD and MAP3K7/TAK1 dependent pathways leading to NF-kappa-B and JNK activation. Ubiquitination of CCS leads to enhancement of its chaperone activity toward its physiologic target, SOD1, rather than proteasomal degradation. Ubiquitination of MAP3K2/MEKK2 and AIFM1 does not lead to proteasomal degradation. Plays a role in copper homeostasis by ubiquitinating COMMD1 and promoting its proteasomal degradation. Can also function as E3 ubiquitin-protein ligase of the NEDD8 conjugation pathway, targeting effector caspases for neddylation and inactivation. Ubiquitinates and therefore mediates the proteasomal degradation of BCL2 in response to apoptosis. Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8. Acts as a positive regulator of Wnt signaling and ubiquitinates TLE1, TLE2, TLE3, TLE4 and AES. Ubiquitination of TLE3 results in inhibition of its interaction with TCF7L2/TCF4 thereby allowing efficient recruitment and binding of the transcriptional coactivator beta-catenin to TCF7L2/TCF4 that is required to initiate a Wnt-specific transcriptional program.
Protein Name E3 Ubiquitin-Protein Ligase Xiap
Baculoviral Iap Repeat-Containing Protein 4
Iap-Like Protein
Ilp
Hilp
Inhibitor Of Apoptosis Protein 3
Iap-3
Hiap-3
Hiap3
Ring-Type E3 Ubiquitin Transferase Xiap
X-Linked Inhibitor Of Apoptosis Protein
X-Linked Iap
Database Links Reactome: R-HSA-111459
Reactome: R-HSA-111463
Reactome: R-HSA-111464
Reactome: R-HSA-111469
Reactome: R-HSA-3769402
Reactome: R-HSA-5213460
Reactome: R-HSA-5357786
Reactome: R-HSA-5357905
Reactome: R-HSA-5357956
Reactome: R-HSA-5675482
Reactome: R-HSA-8948747
Reactome: R-HSA-8948751
Reactome: R-HSA-9627069
Cellular Localisation Cytoplasm
Nucleus
Tle3 Promotes Its Nuclear Localization
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Xiap antibody
Anti-Baculoviral Iap Repeat-Containing Protein 4 antibody
Anti-Iap-Like Protein antibody
Anti-Ilp antibody
Anti-Hilp antibody
Anti-Inhibitor Of Apoptosis Protein 3 antibody
Anti-Iap-3 antibody
Anti-Hiap-3 antibody
Anti-Hiap3 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Xiap antibody
Anti-X-Linked Inhibitor Of Apoptosis Protein antibody
Anti-X-Linked Iap antibody
Anti-XIAP antibody
Anti-API3 antibody
Anti-BIRC4 antibody
Anti-IAP3 antibody

Information sourced from Uniprot.org

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