| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | XIAP |
| Gene ID: | 331 |
| Uniprot ID: | XIAP_HUMAN |
| Immunogen Region: | 198-497 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 198-497 of human XIAP (NP_001158.2). |
| Immunogen Sequence: | VQCFCCGGKLKNWEPCDRAW SEHRRHFPNCFFVLGRNLNI RSESDAVSSDRNFPNSTNLP RNPSMADYEARIFTFGTWIY SVNKEQLARAGFYALGEGDK VKCFHCGGGLTDWKPSEDPW EQHAKWYPGCKYLLEQKGQE YINNIHLTHSLEECLVRTTE KTPSLTRRIDDTIFQNPMVQ EAIRMGFSFKDIKKIMEEKI QISGSNYKSLEVLVADLVNA QKDSMQDESSQTSLQKEIS |
| Tissue Specificity | Expressed in colonic crypts (at protein level). Ubiquitous, except peripheral blood leukocytes. |
| Post Translational Modifications | S-Nitrosylation down-regulates its E3 ubiquitin-protein ligase activity. Autoubiquitinated. Ubiquitinated by TRIM32.leading to proteasomal degradation. |
| Function | Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, copper homeostasis, mitogenic kinase signaling, cell proliferation, as well as cell invasion and metastasis. Acts as a direct caspase inhibitor. Directly bind to the active site pocket of CASP3 and CASP7 and obstructs substrate entry. Inactivates CASP9 by keeping it in a monomeric, inactive state. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and the target proteins for its E3 ubiquitin-protein ligase activity include: RIPK1, RIPK2, MAP3K2/MEKK2, DIABLO/SMAC, AIFM1, CCS, PTEN and BIRC5/survivin. Acts as an important regulator of innate immunity by mediating 'Lys-63'-linked polyubiquitination of RIPK2 downstream of NOD1 and NOD2, thereby transforming RIPK2 into a scaffolding protein for downstream effectors, ultimately leading to activation of the NF-kappa-B and MAP kinases signaling. 'Lys-63'-linked polyubiquitination of RIPK2 also promotes recruitment of the LUBAC complex to RIPK2. Regulates the BMP signaling pathway and the SMAD and MAP3K7/TAK1 dependent pathways leading to NF-kappa-B and JNK activation. Ubiquitination of CCS leads to enhancement of its chaperone activity toward its physiologic target, SOD1, rather than proteasomal degradation. Ubiquitination of MAP3K2/MEKK2 and AIFM1 does not lead to proteasomal degradation. Plays a role in copper homeostasis by ubiquitinating COMMD1 and promoting its proteasomal degradation. Can also function as E3 ubiquitin-protein ligase of the NEDD8 conjugation pathway, targeting effector caspases for neddylation and inactivation. Ubiquitinates and therefore mediates the proteasomal degradation of BCL2 in response to apoptosis. Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8. Acts as a positive regulator of Wnt signaling and ubiquitinates TLE1, TLE2, TLE3, TLE4 and AES. Ubiquitination of TLE3 results in inhibition of its interaction with TCF7L2/TCF4 thereby allowing efficient recruitment and binding of the transcriptional coactivator beta-catenin to TCF7L2/TCF4 that is required to initiate a Wnt-specific transcriptional program. |
| Protein Name | E3 Ubiquitin-Protein Ligase Xiap Baculoviral Iap Repeat-Containing Protein 4 Iap-Like Protein Ilp Hilp Inhibitor Of Apoptosis Protein 3 Iap-3 Hiap-3 Hiap3 Ring-Type E3 Ubiquitin Transferase Xiap X-Linked Inhibitor Of Apoptosis Protein X-Linked Iap |
| Database Links | Reactome: R-HSA-111459 Reactome: R-HSA-111463 Reactome: R-HSA-111464 Reactome: R-HSA-111469 Reactome: R-HSA-3769402 Reactome: R-HSA-5213460 Reactome: R-HSA-5357786 Reactome: R-HSA-5357905 Reactome: R-HSA-5357956 Reactome: R-HSA-5675482 Reactome: R-HSA-8948747 Reactome: R-HSA-8948751 Reactome: R-HSA-9627069 |
| Cellular Localisation | Cytoplasm Nucleus Tle3 Promotes Its Nuclear Localization |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Xiap antibody Anti-Baculoviral Iap Repeat-Containing Protein 4 antibody Anti-Iap-Like Protein antibody Anti-Ilp antibody Anti-Hilp antibody Anti-Inhibitor Of Apoptosis Protein 3 antibody Anti-Iap-3 antibody Anti-Hiap-3 antibody Anti-Hiap3 antibody Anti-Ring-Type E3 Ubiquitin Transferase Xiap antibody Anti-X-Linked Inhibitor Of Apoptosis Protein antibody Anti-X-Linked Iap antibody Anti-XIAP antibody Anti-API3 antibody Anti-BIRC4 antibody Anti-IAP3 antibody |
Information sourced from Uniprot.org