• Western blot analysis of extracts of various cell lines, using Xanthine Oxidase (XDH) (XDH) rabbit monoclonal antibody (STJ11102410) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Confocal imaging of paraffin-embedded Human liver using Xanthine Oxidase (XDH) Rabbit monoclonal antibody (STJ11102410, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry of paraffin-embedded rat kidney using Xanthine Oxidase (XDH) (XDH) rabbit monoclonal antibody (STJ11102410) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Confocal imaging of NIH/3T3 cells using Xanthine Oxidase (XDH) Rabbit monoclonal antibody (STJ11102410, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunohistochemistry of paraffin-embedded human oophoroma using Xanthine Oxidase (XDH) (XDH) rabbit monoclonal antibody (STJ11102410) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Xanthine Oxidase (XDH) in paraffin-embedded mouse kidney using Xanthine Oxidase (XDH) Rabbit monoclonal antibody (STJ11102410) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse brain using Xanthine Oxidase (XDH) (XDH) rabbit monoclonal antibody (STJ11102410) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Xanthine Oxidase (XDH) in paraffin-embedded mouse brain using Xanthine Oxidase (XDH) Rabbit monoclonal antibody (STJ11102410) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH-3T3 cells using Xanthine Oxidase (XDH) (XDH) rabbit monoclonal antibody (STJ11102410) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Xanthine Oxidase (XDH) in paraffin-embedded human liver cancer using Xanthine Oxidase (XDH) Rabbit monoclonal antibody (STJ11102410) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Xanthine Oxidase (XDH) Rabbit monoclonal antibody (STJ11102410) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-XDH antibody (202-293) [S0MR] (STJ11102410)

SKU:
STJ11102410

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Xanthine Oxidase (202-293) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: XDH
Gene ID: 7498
Uniprot ID: XDH_HUMAN
Immunogen Region: 202-293
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 202-293 of human Xanthine Oxidase (XDH) (P47989).
Immunogen Sequence: TPLDPTQEPIFPPELLRLKD TPRKQLRFEGERVTWIQAST LKELLDLKAQHPDAKLVVGN TEIGIEMKFKNMLFPMIVCP AWIPELNSVEHG
Tissue Specificity Detected in milk (at protein level).
Post Translational Modifications Subject to partial proteolysis.this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O). Contains sulfhydryl groups that are easily oxidized (in vitro).this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O).
Function Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro).
Protein Name Xanthine Dehydrogenase/Oxidase Includes - Xanthine Dehydrogenase
Xd - Xanthine Oxidase
Xo
Xanthine Oxidoreductase
Xor
Database Links Reactome: R-HSA-74259
Reactome: R-HSA-8851680
Reactome: R-HSA-9748787
Cellular Localisation Cytoplasm
Peroxisome
Secreted
Alternative Antibody Names Anti-Xanthine Dehydrogenase/Oxidase Includes - Xanthine Dehydrogenase antibody
Anti-Xd - Xanthine Oxidase antibody
Anti-Xo antibody
Anti-Xanthine Oxidoreductase antibody
Anti-Xor antibody
Anti-XDH antibody
Anti-XDHA antibody

Information sourced from Uniprot.org

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