• Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-WWTR1 antibody (STJ191805)

SKU:
STJ191805

Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-WW domain-containing transcription regulator protein 1 is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
ELISA 1:5000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WWTR1
Gene ID: 25937
Uniprot ID: WWTR1_HUMAN
Specificity: WWTR1 Polyclonal Antibody detects endogenous levels of protein.
Immunogen: Synthesized peptide derived from part of the human protein
Post Translational Modifications Phosphorylated by LATS2 and STK3/MST2. Phosphorylation by LATS2 results in creation of 14-3-3 binding sites, retention in the cytoplasm, and functional inactivation. Phosphorylation results in the inhibition of transcriptional coactivation through YWHAZ-mediated nuclear export. Ubiquitinated at Lys-46.leading to proteasomal degradation. Deubiquitinated and stabilized by UCHL1 at Lys-46.leading to osteoclastogenesis inhibition.
Function Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. In conjunction with YAP1, involved in the regulation of TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation. Plays a key role in coupling SMADs to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition.
Protein Name Ww Domain-Containing Transcription Regulator Protein 1
Transcriptional Coactivator With Pdz-Binding Motif
Database Links Reactome: R-HSA-2028269
Reactome: R-HSA-2032785
Reactome: R-HSA-2173795
Reactome: R-HSA-2173796
Reactome: R-HSA-5578768
Reactome: R-HSA-8940973
Reactome: R-HSA-8951671
Reactome: R-HSA-9619665
Cellular Localisation Nucleus
Cytoplasm
Cell Membrane
Concentrates Along Specific Portions Of The Plasma Membrane
And Accumulates In Punctate Nuclear Bodies
When Phosphorylated
Is Retained In The Cytoplasm By Ywhaz
Can Be Retained In The Nucleus By Med15
Localized In The Cytoplasm In Areas Of Epithelial Cell High Density
At Blastocyst Stage Expressed In The Nucleus In Trophectodermal Cells
However Expressed In The Cytoplasm In The Inner Cell Mass
Alternative Antibody Names Anti-Ww Domain-Containing Transcription Regulator Protein 1 antibody
Anti-Transcriptional Coactivator With Pdz-Binding Motif antibody
Anti-WWTR1 antibody
Anti-TAZ antibody

Information sourced from Uniprot.org

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