• Immunofluorescence analysis of NIH-3T3 cells using WWOX Rabbit polyclonal antibody (STJ114526) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using WWOX Rabbit polyclonal antibody (STJ114526) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-WWOX antibody (1-120) (STJ114526)

SKU:
STJ114526

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-WWOX (1-120) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WWOX
Gene ID: 51741
Uniprot ID: WWOX_HUMAN
Immunogen Region: 1-120
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human WWOX (NP_057457.1).
Immunogen Sequence: MAALRYAGLDDTDSEDELPP GWEERTTKDGWVYYANHTEE KTQWEHPKTGKRKRVAGDLP YGWEQETDENGQVFFVDHIN KRTTYLDPRLAFTVDDNPTK PTTRQRYDGSTTAMEILQGR
Tissue Specificity Widely expressed. Strongly expressed in testis, prostate, and ovary. Overexpressed in cancer cell lines. Isoform 5 and isoform 6 may only be expressed in tumor cell lines.
Post Translational Modifications Phosphorylated upon genotoxic stress. Phosphorylation of Tyr-33 regulates interaction with TP53, TP73 and MAPK8. May also regulate proapoptotic activity. Phosphorylation by TNK2 is associated with polyubiquitination and degradation. Ubiquitinated when phosphorylated by TNK2, leading to its degradation.
Function Putative oxidoreductase. Acts as a tumor suppressor and plays a role in apoptosis. Required for normal bone development. May function synergistically with p53/TP53 to control genotoxic stress-induced cell death. Plays a role in TGFB1 signaling and TGFB1-mediated cell death. May also play a role in tumor necrosis factor (TNF)-mediated cell death. Inhibits Wnt signaling, probably by sequestering DVL2 in the cytoplasm.
Protein Name Ww Domain-Containing Oxidoreductase
Fragile Site Fra16d Oxidoreductase
Short Chain Dehydrogenase/Reductase Family 41c Member 1
Database Links Reactome: R-HSA-1251985
Reactome: R-HSA-8866904
Reactome: R-HSA-8866907
Cellular Localisation Cytoplasm
Nucleus
Mitochondrion
Golgi Apparatus
Lysosome
Partially Localizes To The Mitochondria
Translocates To The Nucleus Upon Genotoxic Stress Or Tnf Stimulation
Translocates To The Nucleus In Response To Tgfb1
Isoform 5 And Isoform 6 May Localize In The Nucleus
Localized To The Lysosome Probably Upon Binding To Vopp1
Alternative Antibody Names Anti-Ww Domain-Containing Oxidoreductase antibody
Anti-Fragile Site Fra16d Oxidoreductase antibody
Anti-Short Chain Dehydrogenase/Reductase Family 41c Member 1 antibody
Anti-WWOX antibody
Anti-FOR antibody
Anti-SDR41C1 antibody
Anti-WOX1 antibody

Information sourced from Uniprot.org

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