| Host: | Mouse |
| Applications: | IHC-P |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-WT1 is suitable for use in Immunohistochemistry research applications. |
| Clonality : | Monoclonal |
| Clone ID : | 6F‐H2 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1/Kappa |
| Formulation: | Tris-HCI buffer containing stabilizing protein (BSA) and <0.1% ProClin |
| Purification: | Affinity purified |
| Dilution Range: | 1:100‐200 |
| Storage Instruction: | Store at 2‐8°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate lab‐standardized diluent. Stability after dilution: 7 days at 24°C, 3 months at 2‐8°C, 6months at ‐20°C. |
| Gene Symbol: | WT1 |
| Gene ID: | 7490 |
| Uniprot ID: | WT1_HUMAN |
| Specificity: | Positive control: Malignant mesothelioma |
| Immunogen: | Purified WT protein |
| Tissue Specificity | Expressed in the kidney and a subset of hematopoietic cells. |
| Function | Transcription factor that plays an important role in cellular development and cell survival. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA. |
| Protein Name | Wilms Tumor Protein Wt33 |
| Database Links | Reactome: R-HSA-9690406 Reactome: R-HSA-9831926 |
| Cellular Localisation | Nucleus Nucleolus Cytoplasm Isoforms Lacking The Kts Motif Have A Diffuse Nuclear Location Shuttles Between Nucleus And Cytoplasm Isoform 1: Nucleus Speckle Isoform 4: Nucleus Nucleoplasm |
| Alternative Antibody Names | Anti-Wilms Tumor Protein antibody Anti-Wt33 antibody Anti-WT1 antibody |
Information sourced from Uniprot.org