| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-WRN (1223-1432) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:200-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
WRN |
| Gene ID: |
7486 |
| Uniprot ID: |
WRN_HUMAN |
| Immunogen Region: |
1223-1432 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1223-1432 of human WRN (NP_000544.2). |
| Immunogen Sequence: |
CQTNSVQTDLFSSTKPQEEQ KTSLVAKNKICTLSQSMAIT YSLFQEKKMPLKSIAESRIL PLMTIGMHLSQAVKAGCPLD LERAGLTPEVQKIIADVIRN PPVNSDMSKISLIRMLVPEN IDTYLIHMAIEILKHGPDSG LQPSCDVNKRRCFPGSEEIC SSSKRSKEEVGINTETSSAE RKRRLPVWFAKGSDTSKKLM DKTKRGGLFS |
| Post Translational Modifications | Phosphorylated by PRKDC. |
| Function | Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers.stably associates with foci elements generating binding sites for RP-A. Plays a role in double-strand break repair after gamma-irradiation. |
| Protein Name | Bifunctional 3'-5' Exonuclease/Atp-Dependent Helicase WrnDna Helicase - Recq-Like Type 3Recq Protein-Like 2Werner Syndrome Protein Includes - 3'-5' Exonuclease - Atp-Dependent Helicase |
| Database Links | Reactome: R-HSA-174414Reactome: R-HSA-174437Reactome: R-HSA-3108214Reactome: R-HSA-5685938Reactome: R-HSA-5685942Reactome: R-HSA-5693554Reactome: R-HSA-5693568Reactome: R-HSA-5693579Reactome: R-HSA-5693607Reactome: R-HSA-5693616Reactome: R-HSA-6804756Reactome: R-HSA-69473Reactome: R-HSA-9701192Reactome: R-HSA-9704331Reactome: R-HSA-9704646Reactome: R-HSA-9709570Reactome: R-HSA-9709603 |
| Cellular Localisation | NucleusNucleolusNucleoplasmChromosomeGamma-Irradiation Leads To Its Translocation From Nucleoli To Nucleoplasm And Pml Regulates The Irradiation-Induced Wrn RelocationLocalizes To Dna Damage Sites |
| Alternative Antibody Names | Anti-Bifunctional 3'-5' Exonuclease/Atp-Dependent Helicase Wrn antibodyAnti-Dna Helicase - Recq-Like Type 3 antibodyAnti-Recq Protein-Like 2 antibodyAnti-Werner Syndrome Protein Includes - 3'-5' Exonuclease - Atp-Dependent Helicase antibodyAnti-WRN antibodyAnti-RECQ3 antibodyAnti-RECQL2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
